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Literature summary for 1.1.1.132 extracted from

  • Tatnell, P.J.; Russell, N.J.; Gacesa, P.
    GDP-mannose dehydrogenase is the key regulatory enzyme in alginate biosynthesis in Pseudomonas aeruginosa: evidence from metabolite studies (1994), Microbiology, 140, 1745-1754.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
SDS-PAGE Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
overexpression of tac promoter controlled algD gene containing different plasmids in mucoid strains FRD1 and PAO568 and non-mucoid strains FRD2 and PAO1
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
higher enzyme activities after IPTG induction only for pPT3 containing strains, low GDP-mannuronate production with pMMB66EH only detectable in mucoid strains, higher with pPT3 with increase in cells of mucoid and non-mucoid strains with IPTG, alginate formation occurs only in mucoid strains without significant increase with IPTG, later enzyme of alginate biosynthesis suggested to become rate-limiting Pseudomonas aeruginosa
0.0004
-
+/-0.00008, PAO1/pMMB66EH, mid-exponential, without IPTG Pseudomonas aeruginosa
0.00056
-
+/-0.00024, PAO1/pMMB66EH, early-stationary, with IPTG Pseudomonas aeruginosa
0.00076
-
+/-0.00016, FRD2/pMMB66EH, mid-exponential, with IPTG Pseudomonas aeruginosa
0.00089
-
+/-0.0002, FRD2/pMMB66EH, mid-exponential, without IPTG Pseudomonas aeruginosa
0.00091
-
+/-0.00042, PAO1/pMMB66EH, mid-exponential, with IPTG Pseudomonas aeruginosa
0.00094
-
+/-0.00052, FRD2/pMMB66EH, early-stationary, with IPTG Pseudomonas aeruginosa
0.00106
-
+/-0.00062, PAO1/pMMB66EH, early-stationary, without IPTG Pseudomonas aeruginosa
0.0013
-
+/-0.00004, FRD2/pMMB66EH, early-stationary, without IPTG Pseudomonas aeruginosa
0.00685
-
+/-0.00228, FRD1/pMMB66EH, mid-exponential, with IPTG Pseudomonas aeruginosa
0.00749
-
+/-0.00187, PAO1/pPT3, mid-exponential, without IPTG Pseudomonas aeruginosa
0.00936
-
+/-0.00063, PAO1/pPT3, early-stationary, without IPTG Pseudomonas aeruginosa
0.00968
-
+/-0.00155, FRD1/pMMB66EH, mid-exponential, without IPTG Pseudomonas aeruginosa
0.00992
-
+/-0.00105, PAO568/pMMB66EH, mid-exponential, without IPTG Pseudomonas aeruginosa
0.01097
-
+/-0.00195, FRD2/pPT4, early-stationary, with IPTG Pseudomonas aeruginosa
0.01232
-
+/-0.00397, PAO568/pMMB66EH, mid-exponential, with IPTG Pseudomonas aeruginosa
0.01249
-
+/-0.00113, FRD2/pPT4, early-stationary, without IPTG Pseudomonas aeruginosa
0.01283
-
+/-0.00348, FRD2/pPT3, early-stationary, without IPTG Pseudomonas aeruginosa
0.01312
-
+/-0.0031, FRD2/pPT3, mid-exponential, without IPTG Pseudomonas aeruginosa
0.01401
-
+/-0.00147, PAO1/pPT4, early-stationary, with IPTG Pseudomonas aeruginosa
0.01436
-
+/-0.00303, FRD1/pPT3, mid-exponential, without IPTG Pseudomonas aeruginosa
0.01472
-
+/-0.00135, PAO1/pPT4, early-stationary, without IPTG Pseudomonas aeruginosa
0.01886
-
+/-0.00526, PAO568/pMMB66EH, early-stationary, without IPTG Pseudomonas aeruginosa
0.01891
-
+/-0.0001, PAO568/pPT3, mid-exponential, without IPTG Pseudomonas aeruginosa
0.02322
-
+/-0.00432, PAO568/pMMB66EH, early-stationary, with IPTG Pseudomonas aeruginosa
0.02418
-
+/-0.00628, FRD1/pMMB66EH, early-stationary, without IPTG Pseudomonas aeruginosa
0.02514
-
+/-0.00274, PAO568/pPT4, early-stationary, without IPTG Pseudomonas aeruginosa
0.02542
-
+/-0.00139, PAO568/pPT4, early-stationary, with IPTG Pseudomonas aeruginosa
0.02574
-
+/-0.00581, FRD1/pMMB66EH, early-stationary, with IPTG Pseudomonas aeruginosa
0.02671
-
+/-0.00459, FRD1/pPT4, early-stationary, with IPTG Pseudomonas aeruginosa
0.02905
-
+/-0.00427, FRD1/pPT4, early-stationary, without IPTG Pseudomonas aeruginosa
0.02994
-
+/-0.0066, PAO568/pPT3, early-stationary, without IPTG Pseudomonas aeruginosa
0.03208
-
+/-0.00629, FRD1/pPT3, early-stationary, without IPTG Pseudomonas aeruginosa
0.1259
-
+/-0.02878, PAO1/pPT3, mid-exponential, with IPTG Pseudomonas aeruginosa
0.1364
-
+/-0.01055, FRD2/pPT3, mid-exponential, with IPTG Pseudomonas aeruginosa
0.1627
-
+/-0.03188, PAO568/pPT3, mid-exponential, with IPTG Pseudomonas aeruginosa
0.1758
-
+/-0.03971, FRD1/pPT3, mid-exponential, with IPTG Pseudomonas aeruginosa
0.1919
-
+/-0.00268, PAO568/pPT3, early-stationary, with IPTG Pseudomonas aeruginosa
0.2037
-
+/-0.06998, PAO1/pPT3, early-stationary, with IPTG Pseudomonas aeruginosa
0.2097
-
+/-0.03274, FRD1/pPT3, early-stationary, with IPTG Pseudomonas aeruginosa
0.2196
-
+/-0.05517, FRD2/pPT3, early-stationary, with IPTG Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-mannose + NAD+ + H2O
-
Pseudomonas aeruginosa GDP-mannuronate + NADH
-
ir

Subunits

Subunits Comment Organism
hexamer
-
Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas aeruginosa