Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pectobacterium carotovorum subsp. carotovorum |
Crystallization (Comment) | Organism |
---|---|
in complex with NAD+, hanging drop vapor diffusion method, using 5% (w/v) polyethylene glycol 4000, and 10% (v/v) isopropanol | Pectobacterium carotovorum subsp. carotovorum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0021 | - |
NADH | at pH 7.2 and 45°C | Pectobacterium carotovorum subsp. carotovorum | |
0.005 | - |
NADPH | at pH 7.2 and 45°C | Pectobacterium carotovorum subsp. carotovorum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
4 * 25000, SDS-PAGE | Pectobacterium carotovorum subsp. carotovorum |
90000 | - |
gel filtration | Pectobacterium carotovorum subsp. carotovorum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-didehydro-3-deoxy-D-gluconate + NADH + H+ | Pectobacterium carotovorum subsp. carotovorum | - |
2-dehydro-3-deoxy-D-gluconate + NAD+ | - |
? | |
2,5-didehydro-3-deoxy-D-gluconate + NADPH + H+ | Pectobacterium carotovorum subsp. carotovorum | - |
2-dehydro-3-deoxy-D-gluconate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pectobacterium carotovorum subsp. carotovorum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Toyopearl DEAE-650 M column chromatography, Toyopearl butyl-650 M column chromatography, Q-Sepharose column chromatography, and Superdex 200 pg gel filtration | Pectobacterium carotovorum subsp. carotovorum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-didehydro-3-deoxy-D-gluconate + NADH + H+ | - |
Pectobacterium carotovorum subsp. carotovorum | 2-dehydro-3-deoxy-D-gluconate + NAD+ | - |
? | |
2,5-didehydro-3-deoxy-D-gluconate + NADPH + H+ | - |
Pectobacterium carotovorum subsp. carotovorum | 2-dehydro-3-deoxy-D-gluconate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 25000, SDS-PAGE | Pectobacterium carotovorum subsp. carotovorum |
Synonyms | Comment | Organism |
---|---|---|
2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase | - |
Pectobacterium carotovorum subsp. carotovorum |
2-keto-3-deoxy-D-gluconate dehydrogenase | - |
Pectobacterium carotovorum subsp. carotovorum |
KduD | - |
Pectobacterium carotovorum subsp. carotovorum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Pectobacterium carotovorum subsp. carotovorum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
the enzyme shows more than 70% activity at 40°C for 5 min | Pectobacterium carotovorum subsp. carotovorum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
NADPH | at pH 7.2 and 45°C | Pectobacterium carotovorum subsp. carotovorum | |
0.024 | - |
NADH | at pH 7.2 and 45°C | Pectobacterium carotovorum subsp. carotovorum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Pectobacterium carotovorum subsp. carotovorum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | the enzyme utilizes both NADH and NADPH | Pectobacterium carotovorum subsp. carotovorum | |
NADPH | the enzyme utilizes both NADH and NADPH | Pectobacterium carotovorum subsp. carotovorum |