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Literature summary for 1.1.1.127 extracted from

  • Condemine, G.; Hugouvieux-Cotte-Pattat, N.; Robert-Baudouy, J.
    An enzyme in the pectinolytic pathway of Erwinia chrysanthemi: 2-Keto-3-deoxygluconate oxidoreductase (1984), J. Gen. Microbiol., 130, 2839-2844.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 72% inhibition at 0.01 mM Dickeya chrysanthemi
ATP 30% inhibition for both reduction and oxidation reaction at 1 mM Dickeya chrysanthemi
p-chloromercuribenzoate 50% inhibition at 0.02 mM Dickeya chrysanthemi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
NADH
-
Dickeya chrysanthemi
0.34
-
(4S)-4,6-dihydroxy-2,5-dioxohexanoate
-
Dickeya chrysanthemi
0.4
-
NAD+
-
Dickeya chrysanthemi
7.7
-
2-dehydro-3-deoxy-D-gluconate
-
Dickeya chrysanthemi

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 20% activation at 1 mM Dickeya chrysanthemi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-dehydro-3-deoxy-D-gluconate + NAD+ Dickeya chrysanthemi involved in pectinolysis by some phytopathogenic bacteria (4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
-
r

Organism

Organism UniProt Comment Textmining
Dickeya chrysanthemi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, 2step chromatography Dickeya chrysanthemi

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Dickeya chrysanthemi
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
39
-
-
Dickeya chrysanthemi

Storage Stability

Storage Stability Organism
-20°C, 2 mM DTT, 6 months Dickeya chrysanthemi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydro-3-deoxy-D-gluconate + NAD+ involved in pectinolysis by some phytopathogenic bacteria Dickeya chrysanthemi (4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
-
r
pyruvate + NAD+ 10% of the activity compared to 2-dehydro-3-deoxy-D-gluconate Dickeya chrysanthemi ? + NADH
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Dickeya chrysanthemi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
stable for 15 h Dickeya chrysanthemi
46
-
half-life: 10 min, NADH increases thermal stability Dickeya chrysanthemi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 reduction reaction Dickeya chrysanthemi
10
-
oxidation reaction Dickeya chrysanthemi

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Dickeya chrysanthemi
NADH
-
Dickeya chrysanthemi
NADP+ 53% lower activity than with NAD+ Dickeya chrysanthemi
NADPH 62% lower activity than with NADH Dickeya chrysanthemi