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Literature summary for 1.1.1.124 extracted from
- 5-Keto-D-fructose. IV. A specific reduced nicotinamide adenine dinucleotide phosphate-linked reductase from Gluconobacter cerinus (1966), J. Biol. Chem., 241, 373-378.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose | inhibition of 5-keto-D-frucotse reduction, Ki: 82 mM | Gluconobacter cerinus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
NADPH | - |
Gluconobacter cerinus | |
| 1.3 | - |
NADP+ | - |
Gluconobacter cerinus | |
| 4.2 | - |
5-Keto-D-fructose 1-phosphate | - |
Gluconobacter cerinus | |
| 4.5 | - |
5-keto-D-fructose | - |
Gluconobacter cerinus | |
| 70 | - |
D-fructose | - |
Gluconobacter cerinus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose + NADP+ | Gluconobacter cerinus | predominant formation of D-fructose | 5-dehydro-D-fructose + NADPH | - |
r | |
| D-fructose + NADP+ | Gluconobacter cerinus | highly specific for D-fructose | 5-dehydro-D-fructose + NADPH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gluconobacter cerinus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| high purity | Gluconobacter cerinus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Gluconobacter cerinus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 627 | - |
- |
Gluconobacter cerinus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stable for 3-4 weeks | Gluconobacter cerinus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-keto-D-fructose 1-phosphate + NADPH + H+ | 16% of the rate compared to 5-keto-D-fructose | Gluconobacter cerinus | D-fructose 1-phosphate + NADP+ | - |
? | |
| D-fructose + NADP+ | predominant formation of D-fructose | Gluconobacter cerinus | 5-dehydro-D-fructose + NADPH | - |
r | |
| D-fructose + NADP+ | highly specific for D-fructose | Gluconobacter cerinus | 5-dehydro-D-fructose + NADPH | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
NADPH oxidation in phosphate buffer | Gluconobacter cerinus |
| 7.4 | - |
NADPH oxidation in Tris-HCl buffer | Gluconobacter cerinus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | cannot be replaced by NADH | Gluconobacter cerinus | |
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Release 2023.1 (January 2023)
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