Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-fructose | inhibition of 5-keto-D-frucotse reduction, Ki: 82 mM | Gluconobacter cerinus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
NADPH | - |
Gluconobacter cerinus | |
1.3 | - |
NADP+ | - |
Gluconobacter cerinus | |
4.2 | - |
5-Keto-D-fructose 1-phosphate | - |
Gluconobacter cerinus | |
4.5 | - |
5-keto-D-fructose | - |
Gluconobacter cerinus | |
70 | - |
D-fructose | - |
Gluconobacter cerinus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + NADP+ | Gluconobacter cerinus | predominant formation of D-fructose | 5-dehydro-D-fructose + NADPH | - |
r | |
D-fructose + NADP+ | Gluconobacter cerinus | highly specific for D-fructose | 5-dehydro-D-fructose + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter cerinus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
high purity | Gluconobacter cerinus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Gluconobacter cerinus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
627 | - |
- |
Gluconobacter cerinus |
Storage Stability | Organism |
---|---|
4°C, stable for 3-4 weeks | Gluconobacter cerinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-keto-D-fructose 1-phosphate + NADPH + H+ | 16% of the rate compared to 5-keto-D-fructose | Gluconobacter cerinus | D-fructose 1-phosphate + NADP+ | - |
? | |
D-fructose + NADP+ | predominant formation of D-fructose | Gluconobacter cerinus | 5-dehydro-D-fructose + NADPH | - |
r | |
D-fructose + NADP+ | highly specific for D-fructose | Gluconobacter cerinus | 5-dehydro-D-fructose + NADPH | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
NADPH oxidation in phosphate buffer | Gluconobacter cerinus |
7.4 | - |
NADPH oxidation in Tris-HCl buffer | Gluconobacter cerinus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | cannot be replaced by NADH | Gluconobacter cerinus |