Any feedback?
Literature summary for 1.1.1.124 extracted from
- 5-keto-D-Fructose reductase of Gluconobacter industrius: Purification, crystallization and properties (1981), Agric. Biol. Chem., 45, 863-869.
No PubMed abstract available
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation | Gluconobacter oxydans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| sulfhydryl reagents | - |
Gluconobacter oxydans |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
NADPH | - |
Gluconobacter oxydans | |
| 4.5 | - |
5-keto-D-fructose | - |
Gluconobacter oxydans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
4 * 25000, SDS-PAGE | Gluconobacter oxydans |
| 100000 | - |
gel filtration | Gluconobacter oxydans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose + NADP+ | Gluconobacter oxydans | predominant reduction of 5-keto-D-fructose | 5-dehydro-D-fructose + NADPH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gluconobacter oxydans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| high purity to crystalline form, chromatography techniques | Gluconobacter oxydans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Gluconobacter oxydans | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3 | 5 | D-fructose oxidation | Gluconobacter oxydans |
| 620 | - |
5-keto-D-fructose reduction | Gluconobacter oxydans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose + NADP+ | predominant reduction of 5-keto-D-fructose | Gluconobacter oxydans | 5-dehydro-D-fructose + NADPH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 25000, SDS-PAGE | Gluconobacter oxydans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
5-keto-D-fructose reduction | Gluconobacter oxydans |
| 8.5 | 10 | D-fructose oxidation | Gluconobacter oxydans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | stable between | Gluconobacter oxydans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | highly specific | Gluconobacter oxydans | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
