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Literature summary for 1.1.1.12 extracted from

  • Tiwari, M.K.; Singh, R.K.; Gao, H.; Kim, T.; Chang, S.; Kim, H.S.; Lee, J.K.
    pH-rate profiles of L-arabinitol 4-dehydrogenase from Hypocrea jecorina and its application in L-xylulose production (2014), Bioorg. Med. Chem. Lett., 24, 173-176.
    View publication on PubMed

Application

Application Comment Organism
synthesis rare L-sugar L-xylulose is produced by the enzymatic oxidation of arabinitol to give a yield of approximately 86% Trichoderma reesei

Cloned(Commentary)

Cloned (Comment) Organism
gene lad1, overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Trichoderma reesei

Protein Variants

Protein Variants Comment Organism
H91A site-directed mutagenesis, catalytically inactive mutant Trichoderma reesei
additional information all tested wild-type and mutant enzymes have a similar secondary structure and the global folding of mutant and wild-type HjLAD is similar Trichoderma reesei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
NAD+ pH 9.5, 25°C Trichoderma reesei
0.2
-
NAD+ pH 7.0, 25°C Trichoderma reesei
0.5
-
NAD+ pH 8.0, 25°C Trichoderma reesei
14.5
-
L-arabinitol pH 9.5, 25°C Trichoderma reesei
17.2
-
L-arabinitol pH 8.0, 25°C Trichoderma reesei
18.2
-
L-arabinitol pH 7.0, 25°C Trichoderma reesei

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ the Cys66, His91, Glu92, and Glu176 residues are involved in coordination of catalytic Zn2+ along with a water molecule Trichoderma reesei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, SDS-PAGE Trichoderma reesei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arabinitol + NAD+ Trichoderma reesei
-
L-xylulose + NADH + H+
-
?
L-arabinitol + NAD+ Trichoderma reesei YSM 768
-
L-xylulose + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Trichoderma reesei Q96V44 i.e Trichoderma reesei
-
Trichoderma reesei YSM 768 Q96V44 i.e Trichoderma reesei
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) Trichoderma reesei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24
-
purified recombinant His-tagged enzyme, pH 7.0, 25°C Trichoderma reesei
44
-
purified recombinant His-tagged enzyme, pH 8.0, 25°C Trichoderma reesei
105
-
purified recombinant His-tagged enzyme, pH 9.5, 25°C Trichoderma reesei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arabinitol + NAD+
-
Trichoderma reesei L-xylulose + NADH + H+
-
?
L-arabinitol + NAD+
-
Trichoderma reesei YSM 768 L-xylulose + NADH + H+
-
?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Trichoderma reesei
More homology modeling and docking of L-arabinitol in the substrate-binding pocket of HjLAD, molecular dynamics, overview Trichoderma reesei

Synonyms

Synonyms Comment Organism
LAD
-
Trichoderma reesei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Trichoderma reesei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16
-
L-arabinitol pH 7.0, 25°C Trichoderma reesei
29.3
-
L-arabinitol pH 8.0, 25°C Trichoderma reesei
70
-
L-arabinitol pH 9.5, 25°C Trichoderma reesei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
Trichoderma reesei

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5 pH-profile, overview Trichoderma reesei

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Trichoderma reesei

General Information

General Information Comment Organism
additional information homology modeling and docking of L-arabinitol in the substrate-binding pocket of HjLAD suggesting routes of hydride transfer, where the key amino acid residues comprise the core region, molecular dynamics, overview Trichoderma reesei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.88
-
L-arabinitol pH 7.0, 25°C Trichoderma reesei
1.7
-
L-arabinitol pH 8.0, 25°C Trichoderma reesei
4.83
-
L-arabinitol pH 9.5, 25°C Trichoderma reesei