Application | Comment | Organism |
---|---|---|
synthesis | rare L-sugar L-xylulose is produced by the enzymatic oxidation of arabinitol to give a yield of approximately 86% | Trichoderma reesei |
Cloned (Comment) | Organism |
---|---|
gene lad1, overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Trichoderma reesei |
Protein Variants | Comment | Organism |
---|---|---|
H91A | site-directed mutagenesis, catalytically inactive mutant | Trichoderma reesei |
additional information | all tested wild-type and mutant enzymes have a similar secondary structure and the global folding of mutant and wild-type HjLAD is similar | Trichoderma reesei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
NAD+ | pH 9.5, 25°C | Trichoderma reesei | |
0.2 | - |
NAD+ | pH 7.0, 25°C | Trichoderma reesei | |
0.5 | - |
NAD+ | pH 8.0, 25°C | Trichoderma reesei | |
14.5 | - |
L-arabinitol | pH 9.5, 25°C | Trichoderma reesei | |
17.2 | - |
L-arabinitol | pH 8.0, 25°C | Trichoderma reesei | |
18.2 | - |
L-arabinitol | pH 7.0, 25°C | Trichoderma reesei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | the Cys66, His91, Glu92, and Glu176 residues are involved in coordination of catalytic Zn2+ along with a water molecule | Trichoderma reesei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Trichoderma reesei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arabinitol + NAD+ | Trichoderma reesei | - |
L-xylulose + NADH + H+ | - |
? | |
L-arabinitol + NAD+ | Trichoderma reesei YSM 768 | - |
L-xylulose + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma reesei | Q96V44 | i.e Trichoderma reesei | - |
Trichoderma reesei YSM 768 | Q96V44 | i.e Trichoderma reesei | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) | Trichoderma reesei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
24 | - |
purified recombinant His-tagged enzyme, pH 7.0, 25°C | Trichoderma reesei |
44 | - |
purified recombinant His-tagged enzyme, pH 8.0, 25°C | Trichoderma reesei |
105 | - |
purified recombinant His-tagged enzyme, pH 9.5, 25°C | Trichoderma reesei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arabinitol + NAD+ | - |
Trichoderma reesei | L-xylulose + NADH + H+ | - |
? | |
L-arabinitol + NAD+ | - |
Trichoderma reesei YSM 768 | L-xylulose + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Trichoderma reesei |
More | homology modeling and docking of L-arabinitol in the substrate-binding pocket of HjLAD, molecular dynamics, overview | Trichoderma reesei |
Synonyms | Comment | Organism |
---|---|---|
LAD | - |
Trichoderma reesei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Trichoderma reesei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16 | - |
L-arabinitol | pH 7.0, 25°C | Trichoderma reesei | |
29.3 | - |
L-arabinitol | pH 8.0, 25°C | Trichoderma reesei | |
70 | - |
L-arabinitol | pH 9.5, 25°C | Trichoderma reesei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Trichoderma reesei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.5 | pH-profile, overview | Trichoderma reesei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Trichoderma reesei |
General Information | Comment | Organism |
---|---|---|
additional information | homology modeling and docking of L-arabinitol in the substrate-binding pocket of HjLAD suggesting routes of hydride transfer, where the key amino acid residues comprise the core region, molecular dynamics, overview | Trichoderma reesei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.88 | - |
L-arabinitol | pH 7.0, 25°C | Trichoderma reesei | |
1.7 | - |
L-arabinitol | pH 8.0, 25°C | Trichoderma reesei | |
4.83 | - |
L-arabinitol | pH 9.5, 25°C | Trichoderma reesei |