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Literature summary for 1.1.1.12 extracted from

  • Sukpipat, W.; Komeda, H.; Prasertsan, P.; Asano, Y.
    Purification and characterization of xylitol dehydrogenase with L-arabitol dehydrogenase activity from the newly isolated pentose-fermenting yeast Meyerozyma caribbica 5XY2 (2016), J. Biosci. Bioeng., 123, 20-27.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene xyl2, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain JM109, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Meyerozyma caribbica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
31.1
-
L-arabinitol recombinant enzyme, pH 7.0, 30°C Meyerozyma caribbica

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc metalloenzyme Meyerozyma caribbica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
-
Meyerozyma caribbica
195000
-
native enzyme, gel filtration Meyerozyma caribbica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arabinitol + NAD+ Meyerozyma caribbica
-
L-xylulose + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Meyerozyma caribbica A0A1B4XTS0 pentose-fermenting strain isolated from an alcohol fermentation starter in Thailand
-
Meyerozyma caribbica 5XY2 A0A1B4XTS0 pentose-fermenting strain isolated from an alcohol fermentation starter in Thailand
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by ammonium sulfate fractionation, anion exchange chromatography, hydrophobic interation chromatography, and another different step of anion exchange chromatography, followed by gel filtration. Recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography Meyerozyma caribbica

Source Tissue

Source Tissue Comment Organism Textmining
cell culture strain 5XY2 catabolizes L-arabinose as well as D-glucose and D-xylose in fermentation Meyerozyma caribbica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.9
-
purified native enzyme, pH 7.0, 40°C, substrates L-arabinitol and NAD+ Meyerozyma caribbica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arabinitol + NAD+
-
Meyerozyma caribbica L-xylulose + NADH + H+
-
?
additional information the enzyme exhibits broad specificity to polyols, such as xylitol, D-sorbitol, ribitol (cf. EC 1.1.1.56), and L-arabitol. Xylitol is the preferred substrate, but native and recombinant enzyme McXDH exhibits relative activities toward L-arabinitol of approximately 20% that toward xylitol Meyerozyma caribbica ?
-
?
additional information the enzyme exhibits broad specificity to polyols, such as xylitol, D-sorbitol, ribitol (cf. EC 1.1.1.56), and L-arabitol. Xylitol is the preferred substrate, but native and recombinant enzyme McXDH exhibits relative activities toward L-arabinitol of approximately 20% that toward xylitol Meyerozyma caribbica 5XY2 ?
-
?

Subunits

Subunits Comment Organism
homopentamer 5 * 39000, SDS-PAGE Meyerozyma caribbica

Synonyms

Synonyms Comment Organism
L-arabitol dehydrogenase
-
Meyerozyma caribbica
LAD
-
Meyerozyma caribbica
XYL2
-
Meyerozyma caribbica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Meyerozyma caribbica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 55 activity range, inactive at 60°C Meyerozyma caribbica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.108
-
L-arabinitol recombinant enzyme, pH 7.0, 30°C Meyerozyma caribbica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
recombinant enzyme Meyerozyma caribbica
9.5
-
native enzyme Meyerozyma caribbica

pH Range

pH Minimum pH Maximum Comment Organism
4 11 activity range, inactive above or below Meyerozyma caribbica

Cofactor

Cofactor Comment Organism Structure
NAD+ preferred cofactor Meyerozyma caribbica
NADP+ low activity Meyerozyma caribbica

General Information

General Information Comment Organism
evolution the enzyme belongs to a medium-chain dehydrogenase/reductase (MDR) superfamily and a subfamily of polyol dehydrogenase, PDH Meyerozyma caribbica