Cloned (Comment) | Organism |
---|---|
gene xyl2, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain JM109, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Meyerozyma caribbica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
31.1 | - |
L-arabinitol | recombinant enzyme, pH 7.0, 30°C | Meyerozyma caribbica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | zinc metalloenzyme | Meyerozyma caribbica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
- |
Meyerozyma caribbica |
195000 | - |
native enzyme, gel filtration | Meyerozyma caribbica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arabinitol + NAD+ | Meyerozyma caribbica | - |
L-xylulose + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Meyerozyma caribbica | A0A1B4XTS0 | pentose-fermenting strain isolated from an alcohol fermentation starter in Thailand | - |
Meyerozyma caribbica 5XY2 | A0A1B4XTS0 | pentose-fermenting strain isolated from an alcohol fermentation starter in Thailand | - |
Purification (Comment) | Organism |
---|---|
native enzyme by ammonium sulfate fractionation, anion exchange chromatography, hydrophobic interation chromatography, and another different step of anion exchange chromatography, followed by gel filtration. Recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography | Meyerozyma caribbica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | strain 5XY2 catabolizes L-arabinose as well as D-glucose and D-xylose in fermentation | Meyerozyma caribbica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.9 | - |
purified native enzyme, pH 7.0, 40°C, substrates L-arabinitol and NAD+ | Meyerozyma caribbica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arabinitol + NAD+ | - |
Meyerozyma caribbica | L-xylulose + NADH + H+ | - |
? | |
additional information | the enzyme exhibits broad specificity to polyols, such as xylitol, D-sorbitol, ribitol (cf. EC 1.1.1.56), and L-arabitol. Xylitol is the preferred substrate, but native and recombinant enzyme McXDH exhibits relative activities toward L-arabinitol of approximately 20% that toward xylitol | Meyerozyma caribbica | ? | - |
? | |
additional information | the enzyme exhibits broad specificity to polyols, such as xylitol, D-sorbitol, ribitol (cf. EC 1.1.1.56), and L-arabitol. Xylitol is the preferred substrate, but native and recombinant enzyme McXDH exhibits relative activities toward L-arabinitol of approximately 20% that toward xylitol | Meyerozyma caribbica 5XY2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homopentamer | 5 * 39000, SDS-PAGE | Meyerozyma caribbica |
Synonyms | Comment | Organism |
---|---|---|
L-arabitol dehydrogenase | - |
Meyerozyma caribbica |
LAD | - |
Meyerozyma caribbica |
XYL2 | - |
Meyerozyma caribbica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Meyerozyma caribbica |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 55 | activity range, inactive at 60°C | Meyerozyma caribbica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.108 | - |
L-arabinitol | recombinant enzyme, pH 7.0, 30°C | Meyerozyma caribbica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
recombinant enzyme | Meyerozyma caribbica |
9.5 | - |
native enzyme | Meyerozyma caribbica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | activity range, inactive above or below | Meyerozyma caribbica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | preferred cofactor | Meyerozyma caribbica | |
NADP+ | low activity | Meyerozyma caribbica |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to a medium-chain dehydrogenase/reductase (MDR) superfamily and a subfamily of polyol dehydrogenase, PDH | Meyerozyma caribbica |