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Literature summary for 1.1.1.119 extracted from

  • Esclapez, J.; Britton, K.L.; Baker, P.J.; Fisher, M.; Pire, C.; Ferrer, J.; Bonete, M.J.; Rice, D.W.
    Crystallization and preliminary X-ray analysis of binary and ternary complexes of Haloferax mediterranei glucose dehydrogenase (2005), Acta Crystallogr. Sect. F, 61, 743-746.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Zn2+
-
Haloferax mediterranei

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour diffusion Haloferax mediterranei
hanging-drop vapour-diffusion method at 17°C, crystallization of a range of binary and ternary complexes of the wild-type and a D38C mutant protein. For most of the complexes, crystals belonging to space group I222 are obtained using sodium/potassium citrate as a precipitant. For the binary and non-productive ternary complexes with NADPH/Zn, it is necessary to replace the citrate with 2-methyl-2,4-pentanediol. Despite the radical change in conditions, the crystals thus formed are isomorphous Haloferax mediterranei

Protein Variants

Protein Variants Comment Organism
D38C crystallization of a range of binary and ternary complexes of the wild-type and a D38C mutant protein Haloferax mediterranei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19500
-
2 * 19500 Haloferax mediterranei
39000
-
-
Haloferax mediterranei

Organism

Organism UniProt Comment Textmining
Haloferax mediterranei
-
strain ATCC 33500/R4
-
Haloferax mediterranei Q977U7
-
-
Haloferax mediterranei DSM 1411 Q977U7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Haloferax mediterranei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-galactose + NADP+
-
Haloferax mediterranei D-galactono-1,5-lactone + NADPH
-
?

Subunits

Subunits Comment Organism
dimer 2 * 19500 Haloferax mediterranei

Synonyms

Synonyms Comment Organism
GlcDH
-
Haloferax mediterranei
glucose dehydrogenase
-
Haloferax mediterranei

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Haloferax mediterranei