Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
apo and NADPH-complexed enzyme forms, hanging drop vapor diffusion method, using 25% (w/v) polyethylene glycol 3350, 0.1 M HEPES pH 7.5 | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabinose + NADP+ | Saccharomyces cerevisiae | - |
D-arabinono-1,4-lactone + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P38115 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabinose + NADP+ | - |
Saccharomyces cerevisiae | D-arabinono-1,4-lactone + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
ARA1 | - |
Saccharomyces cerevisiae |
D-arabinose dehydrogenase | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Saccharomyces cerevisiae |