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Literature summary for 1.1.1.116 extracted from
- NAD(+)-specific D-arabinose dehydrogenase and its contribution to erythroascorbic acid production in Saccharomyces cerevisiae (2006), FEBS Lett., 580, 6428-6434.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene YMR041c or ARA2, phylogenetic tree, expression of His6-tagged Ara2p in Escherichia coli | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | ARA2 gene disruption leads to complete loss of NAD+-specific D-arabinose dehydrogenase activity | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-arabinose | substrate inhibition of Ara2p at concentrations above 5 mM | Saccharomyces cerevisiae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.78 | - |
D-arabinose | pH 8.2, 25°C, recombinant His-tagged Ara2p | Saccharomyces cerevisiae | |
| 4.1 | - |
NAD+ | pH 8.2, 25°C, recombinant His-tagged Ara2p | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
recombinant Ara2p, gel filtration | Saccharomyces cerevisiae |
| 42400 | - |
1 * 42400, recombinant Ara2p, SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-arabinose + NAD+ | Saccharomyces cerevisiae | the enzyme, mainly Ara2p not Ara1p, is involved in the biosynthesis of D-erythroascorbic acid, the five-carbon analog of ascorbic acid, from exogeneous D-arabinose | D-arabinono-1,4-lactone + NADH | - |
? | |
| D-arabinose + NAD+ | Saccharomyces cerevisiae YPH250 | the enzyme, mainly Ara2p not Ara1p, is involved in the biosynthesis of D-erythroascorbic acid, the five-carbon analog of ascorbic acid, from exogeneous D-arabinose | D-arabinono-1,4-lactone + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | Q04212 | ARA2; gene YMR041c or ARA2, isozyme Ara2p | - |
| Saccharomyces cerevisiae YPH250 | Q04212 | ARA2; gene YMR041c or ARA2, isozyme Ara2p | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged Ara2p from Escherichia coli by anion exchange and nickel affinity chromatography to homogeneity | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00071 | - |
NAD+-dependent Ara2p reaction in presence of 200 mM D-arabinose, native cell extract | Saccharomyces cerevisiae |
| 1.8 | - |
purified recombinant His-tagged ARA2p | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-arabinose + NAD+ | the enzyme, mainly Ara2p not Ara1p, is involved in the biosynthesis of D-erythroascorbic acid, the five-carbon analog of ascorbic acid, from exogeneous D-arabinose | Saccharomyces cerevisiae | D-arabinono-1,4-lactone + NADH | - |
? | |
| D-arabinose + NAD+ | preferred sugar substrate | Saccharomyces cerevisiae | D-arabinono-1,4-lactone + NADH | - |
? | |
| D-arabinose + NAD+ | the enzyme, mainly Ara2p not Ara1p, is involved in the biosynthesis of D-erythroascorbic acid, the five-carbon analog of ascorbic acid, from exogeneous D-arabinose | Saccharomyces cerevisiae YPH250 | D-arabinono-1,4-lactone + NADH | - |
? | |
| D-arabinose + NAD+ | preferred sugar substrate | Saccharomyces cerevisiae YPH250 | D-arabinono-1,4-lactone + NADH | - |
? | |
| L-fucose + NAD+ | - |
Saccharomyces cerevisiae | ? | - |
? | |
| L-fucose + NAD+ | - |
Saccharomyces cerevisiae YPH250 | ? | - |
? | |
| L-galactose + NAD+ | - |
Saccharomyces cerevisiae | ? | - |
? | |
| L-galactose + NAD+ | - |
Saccharomyces cerevisiae YPH250 | ? | - |
? | |
| L-xylose + NAD+ | - |
Saccharomyces cerevisiae | ? | - |
? | |
| additional information | ARa2p shows a broad sugar substrate specificity, but prefers D-arabinose | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | ARa2p shows a broad sugar substrate specificity, but prefers D-arabinose | Saccharomyces cerevisiae YPH250 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 42400, recombinant Ara2p, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ARA | - |
Saccharomyces cerevisiae |
| Ara2p | - |
Saccharomyces cerevisiae |
| NAD(+)-specific D-arabinose dehydrogenase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
- |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | 20% of maximal activity at pH 6.5 and pH 9.0, pH profile | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | Ara2p is specific for NAD+, while Ara1p utilizes NADP+ | Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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