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Literature summary for 1.1.1.116 extracted from
- Production of L-ascorbic acid by metabolically engineered Saccharomyces cerevisiae and Zygosaccharomyces bailii (2004), Appl. Environ. Microbiol., 70, 6086-6091.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | production of L-ascorbic acid and secretion into culture medium by overexpression of enzyme and arabinone-1,4-lactone oxidase in Saccharomyces cerevisiae and Zygosaccharomyces bailii | Saccharomyces cerevisiae |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ARA1, co-overexpression of the enzyme and the D-arabinono-1,4-lactone oxidase and/or L-galactono-1,4-lactone dehydrogenase in Saccharomyces cerevisiae strains result in overproduction and accumulation of vitamin C in the culture medium, while overexpression of the D-arabinose dehydrogenase alone does not alter the L-ascorbate content, overview | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-arabinose + NAD+ | Saccharomyces cerevisiae | - |
D-arabinono-1,4-lactone + NADH | - |
? |  |
| D-arabinose + NAD+ | Saccharomyces cerevisiae GRF18U | - |
D-arabinono-1,4-lactone + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
recombinant enzyme overexpressed in Saccharomyces cerevisiae and Zygosaccharomyces bailii | - |
| Saccharomyces cerevisiae | - |
gene ARA1 | - |
| Saccharomyces cerevisiae GRF18U | - |
gene ARA1 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-arabinose + NAD+ | - |
Saccharomyces cerevisiae | D-arabinono-1,4-lactone + NADH | - |
? | |
| D-arabinose + NAD+ | - |
Saccharomyces cerevisiae GRF18U | D-arabinono-1,4-lactone + NADH | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ARA | - |
Saccharomyces cerevisiae |
| D-arabinose dehydrogenase | - |
Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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