Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mesorhizobium loti |
Crystallization (Comment) | Organism |
---|---|
recombinant selenomethionine-substituted enzyme, crystallized by the sitting-drop vapour-diffusion method using PEG 4000 as precipitant, in the presence of 0.45 mM NAD+. The crystals diffract to 2.9 A resolution and belong to the monoclinic space group P21 | Mesorhizobium loti |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
x * 26000, SDS-PAGE | Mesorhizobium loti |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesorhizobium loti | Q988B7 | - |
- |
Mesorhizobium loti MAFF303099 | Q988B7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Mesorhizobium loti |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
103.9 | - |
selenomethionine-substituted enzyme, 30°C, pH 9.2 | Mesorhizobium loti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyridoxal + NAD+ | - |
Mesorhizobium loti | 4-pyridoxolactone + NADH + H+ | - |
? | |
pyridoxal + NAD+ | - |
Mesorhizobium loti MAFF303099 | 4-pyridoxolactone + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26000, SDS-PAGE | Mesorhizobium loti |
Synonyms | Comment | Organism |
---|---|---|
PLDH | - |
Mesorhizobium loti |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mesorhizobium loti |