Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.107 extracted from

  • Yokochi, N.; Nishimura, S.; Yoshikane, Y.; Ohnishi, K.; Yagi, T.
    Identification of a new tetrameric pyridoxal 4-dehydrogenase as the second enzyme in the degradation pathway for pyridoxine in a nitrogen-fixing symbiotic bacterium, Mesorhizobium loti (2006), Arch. Biochem. Biophys., 452, 1-8.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mesorhizobium loti
recombinant enzyme expressed in Escherichia coli Mesorhizobium loti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.091
-
pyridoxal pH 9.2, 30°C Mesorhizobium loti
0.091
-
pyridoxal 30°C, pH 9.2 Mesorhizobium loti
0.28
-
NAD+ pH 9.2, 30°C Mesorhizobium loti
0.28
-
NAD+ 30°C, pH 9.2 Mesorhizobium loti

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25531
-
4 * 25531, calculated from sequence Mesorhizobium loti
26000
-
4 * 26000, SDS-PAGE Mesorhizobium loti
26000
-
4 * 26000 Mesorhizobium loti
97000
-
gel filtration Mesorhizobium loti
98000
-
non-denaturing PAGE Mesorhizobium loti

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyridoxal + NAD+ Mesorhizobium loti PLDH is essential for assimilation of vitamin B6 compounds and is the second step enzyme in the degradation pathway for pyridoxine 4-pyridoxolactone + NADH + H+
-
?
pyridoxal + NAD+ Mesorhizobium loti PLDH is essential for the assimilation of vitamin B6 compounds and the second step enzyme in their degradation pathway 4-pyridoxolactone + NADH + H+
-
ir
pyridoxal + NAD+ Mesorhizobium loti MAFF303099 PLDH is essential for assimilation of vitamin B6 compounds and is the second step enzyme in the degradation pathway for pyridoxine 4-pyridoxolactone + NADH + H+
-
?
pyridoxal + NAD+ Mesorhizobium loti MAFF303099 PLDH is essential for the assimilation of vitamin B6 compounds and the second step enzyme in their degradation pathway 4-pyridoxolactone + NADH + H+
-
ir

Organism

Organism UniProt Comment Textmining
Mesorhizobium loti
-
-
-
Mesorhizobium loti
-
MAFF303099
-
Mesorhizobium loti MAFF303099
-
MAFF303099
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Mesorhizobium loti

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
88
-
-
Mesorhizobium loti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme shows less than 0.01% of the activity of pyridoxal toward pyridoxal 5'-phosphate (0.5 mM), pyridoxine (0.8 mM), and pyridoxamine, pyridoxamine 5'-phosphate, 4-phthalaldehyde, 2-nitrobenzaldehyde, 3-nitrobenzaldehyde, 4-nitrobenzaldehyde, pyridine, formaldehyde, and 2-carboxybenzaldehyde (1.0 mM each). The enzyme shows less than 0.01% of the activity of pyridoxal toward DL-glyceraldehyde, D-xylose, L-xylose, D-ribose, D-glucose, D-arabinose, L-arabinose, L-fucose, and D-galactose (250 mM each) Mesorhizobium loti ?
-
?
additional information the enzyme shows less than 0.01% of the activity of pyridoxal toward pyridoxal 5'-phosphate (0.5 mM), pyridoxine (0.8 mM), and pyridoxamine, pyridoxamine 5'-phosphate, 4-phthalaldehyde, 2-nitrobenzaldehyde, 3-nitrobenzaldehyde, 4-nitrobenzaldehyde, pyridine, formaldehyde, and 2-carboxybenzaldehyde (1.0 mM each). The enzyme shows less than 0.01% of the activity of pyridoxal toward DL-glyceraldehyde, D-xylose, L-xylose, D-ribose, D-glucose, D-arabinose, L-arabinose, L-fucose, and D-galactose (250 mM each) Mesorhizobium loti MAFF303099 ?
-
?
pyridoxal + NAD+ PLDH is essential for assimilation of vitamin B6 compounds and is the second step enzyme in the degradation pathway for pyridoxine Mesorhizobium loti 4-pyridoxolactone + NADH + H+
-
?
pyridoxal + NAD+ PLDH is essential for the assimilation of vitamin B6 compounds and the second step enzyme in their degradation pathway Mesorhizobium loti 4-pyridoxolactone + NADH + H+
-
ir
pyridoxal + NAD+ very low activity toward NADP+. 0.16% of the maximal activity obtained with NAD+ is observed when 5.0 mM NADP+ is used. The enzyme does not catalyze the reduction of 4-pyridoxolactone with NADH + H+ to pyridoxal even when a 100fold higher concentration of the enzyme is used for the reverse reaction Mesorhizobium loti 4-pyridoxolactone + NADH + H+
-
ir
pyridoxal + NAD+ PLDH is essential for assimilation of vitamin B6 compounds and is the second step enzyme in the degradation pathway for pyridoxine Mesorhizobium loti MAFF303099 4-pyridoxolactone + NADH + H+
-
?
pyridoxal + NAD+ PLDH is essential for the assimilation of vitamin B6 compounds and the second step enzyme in their degradation pathway Mesorhizobium loti MAFF303099 4-pyridoxolactone + NADH + H+
-
ir
pyridoxal + NAD+ very low activity toward NADP+. 0.16% of the maximal activity obtained with NAD+ is observed when 5.0 mM NADP+ is used. The enzyme does not catalyze the reduction of 4-pyridoxolactone with NADH + H+ to pyridoxal even when a 100fold higher concentration of the enzyme is used for the reverse reaction Mesorhizobium loti MAFF303099 4-pyridoxolactone + NADH + H+
-
ir

Subunits

Subunits Comment Organism
tetramer 4 * 26000, SDS-PAGE Mesorhizobium loti
tetramer 4 * 26000 Mesorhizobium loti
tetramer 4 * 25531, calculated from sequence Mesorhizobium loti

Synonyms

Synonyms Comment Organism
PLDH
-
Mesorhizobium loti

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Mesorhizobium loti

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 55 30°C: 37% of maximal activity, 55°C: 33% of maximal activity Mesorhizobium loti

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
10 min, stable below Mesorhizobium loti
50
-
10 min, 76% of original activity Mesorhizobium loti
60
-
10 min, 1.3% of original activity Mesorhizobium loti

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
136
-
NAD+ pH 9.2, 30°C Mesorhizobium loti
136
-
NAD+ 30°C, pH 9.2 Mesorhizobium loti
149
-
pyridoxal pH 9.2, 30°C Mesorhizobium loti
149
-
pyridoxal 30°C, pH 9.2 Mesorhizobium loti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.2
-
-
Mesorhizobium loti
9.2
-
50 mM CHES-NaOH buffer Mesorhizobium loti

pH Range

pH Minimum pH Maximum Comment Organism
8.6 10 pH 8.6: 66% of maximal activity, pH 10.0: 57% of maximal activity, 50 mM CHES-NaOH buffer Mesorhizobium loti

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Mesorhizobium loti