Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mesorhizobium loti |
recombinant enzyme expressed in Escherichia coli | Mesorhizobium loti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.091 | - |
pyridoxal | pH 9.2, 30°C | Mesorhizobium loti | |
0.091 | - |
pyridoxal | 30°C, pH 9.2 | Mesorhizobium loti | |
0.28 | - |
NAD+ | pH 9.2, 30°C | Mesorhizobium loti | |
0.28 | - |
NAD+ | 30°C, pH 9.2 | Mesorhizobium loti |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25531 | - |
4 * 25531, calculated from sequence | Mesorhizobium loti |
26000 | - |
4 * 26000, SDS-PAGE | Mesorhizobium loti |
26000 | - |
4 * 26000 | Mesorhizobium loti |
97000 | - |
gel filtration | Mesorhizobium loti |
98000 | - |
non-denaturing PAGE | Mesorhizobium loti |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyridoxal + NAD+ | Mesorhizobium loti | PLDH is essential for assimilation of vitamin B6 compounds and is the second step enzyme in the degradation pathway for pyridoxine | 4-pyridoxolactone + NADH + H+ | - |
? | |
pyridoxal + NAD+ | Mesorhizobium loti | PLDH is essential for the assimilation of vitamin B6 compounds and the second step enzyme in their degradation pathway | 4-pyridoxolactone + NADH + H+ | - |
ir | |
pyridoxal + NAD+ | Mesorhizobium loti MAFF303099 | PLDH is essential for assimilation of vitamin B6 compounds and is the second step enzyme in the degradation pathway for pyridoxine | 4-pyridoxolactone + NADH + H+ | - |
? | |
pyridoxal + NAD+ | Mesorhizobium loti MAFF303099 | PLDH is essential for the assimilation of vitamin B6 compounds and the second step enzyme in their degradation pathway | 4-pyridoxolactone + NADH + H+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesorhizobium loti | - |
- |
- |
Mesorhizobium loti | - |
MAFF303099 | - |
Mesorhizobium loti MAFF303099 | - |
MAFF303099 | - |
Purification (Comment) | Organism |
---|---|
recombinant | Mesorhizobium loti |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
88 | - |
- |
Mesorhizobium loti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme shows less than 0.01% of the activity of pyridoxal toward pyridoxal 5'-phosphate (0.5 mM), pyridoxine (0.8 mM), and pyridoxamine, pyridoxamine 5'-phosphate, 4-phthalaldehyde, 2-nitrobenzaldehyde, 3-nitrobenzaldehyde, 4-nitrobenzaldehyde, pyridine, formaldehyde, and 2-carboxybenzaldehyde (1.0 mM each). The enzyme shows less than 0.01% of the activity of pyridoxal toward DL-glyceraldehyde, D-xylose, L-xylose, D-ribose, D-glucose, D-arabinose, L-arabinose, L-fucose, and D-galactose (250 mM each) | Mesorhizobium loti | ? | - |
? | |
additional information | the enzyme shows less than 0.01% of the activity of pyridoxal toward pyridoxal 5'-phosphate (0.5 mM), pyridoxine (0.8 mM), and pyridoxamine, pyridoxamine 5'-phosphate, 4-phthalaldehyde, 2-nitrobenzaldehyde, 3-nitrobenzaldehyde, 4-nitrobenzaldehyde, pyridine, formaldehyde, and 2-carboxybenzaldehyde (1.0 mM each). The enzyme shows less than 0.01% of the activity of pyridoxal toward DL-glyceraldehyde, D-xylose, L-xylose, D-ribose, D-glucose, D-arabinose, L-arabinose, L-fucose, and D-galactose (250 mM each) | Mesorhizobium loti MAFF303099 | ? | - |
? | |
pyridoxal + NAD+ | PLDH is essential for assimilation of vitamin B6 compounds and is the second step enzyme in the degradation pathway for pyridoxine | Mesorhizobium loti | 4-pyridoxolactone + NADH + H+ | - |
? | |
pyridoxal + NAD+ | PLDH is essential for the assimilation of vitamin B6 compounds and the second step enzyme in their degradation pathway | Mesorhizobium loti | 4-pyridoxolactone + NADH + H+ | - |
ir | |
pyridoxal + NAD+ | very low activity toward NADP+. 0.16% of the maximal activity obtained with NAD+ is observed when 5.0 mM NADP+ is used. The enzyme does not catalyze the reduction of 4-pyridoxolactone with NADH + H+ to pyridoxal even when a 100fold higher concentration of the enzyme is used for the reverse reaction | Mesorhizobium loti | 4-pyridoxolactone + NADH + H+ | - |
ir | |
pyridoxal + NAD+ | PLDH is essential for assimilation of vitamin B6 compounds and is the second step enzyme in the degradation pathway for pyridoxine | Mesorhizobium loti MAFF303099 | 4-pyridoxolactone + NADH + H+ | - |
? | |
pyridoxal + NAD+ | PLDH is essential for the assimilation of vitamin B6 compounds and the second step enzyme in their degradation pathway | Mesorhizobium loti MAFF303099 | 4-pyridoxolactone + NADH + H+ | - |
ir | |
pyridoxal + NAD+ | very low activity toward NADP+. 0.16% of the maximal activity obtained with NAD+ is observed when 5.0 mM NADP+ is used. The enzyme does not catalyze the reduction of 4-pyridoxolactone with NADH + H+ to pyridoxal even when a 100fold higher concentration of the enzyme is used for the reverse reaction | Mesorhizobium loti MAFF303099 | 4-pyridoxolactone + NADH + H+ | - |
ir |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 26000, SDS-PAGE | Mesorhizobium loti |
tetramer | 4 * 26000 | Mesorhizobium loti |
tetramer | 4 * 25531, calculated from sequence | Mesorhizobium loti |
Synonyms | Comment | Organism |
---|---|---|
PLDH | - |
Mesorhizobium loti |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Mesorhizobium loti |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | 30°C: 37% of maximal activity, 55°C: 33% of maximal activity | Mesorhizobium loti |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
10 min, stable below | Mesorhizobium loti |
50 | - |
10 min, 76% of original activity | Mesorhizobium loti |
60 | - |
10 min, 1.3% of original activity | Mesorhizobium loti |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
136 | - |
NAD+ | pH 9.2, 30°C | Mesorhizobium loti | |
136 | - |
NAD+ | 30°C, pH 9.2 | Mesorhizobium loti | |
149 | - |
pyridoxal | pH 9.2, 30°C | Mesorhizobium loti | |
149 | - |
pyridoxal | 30°C, pH 9.2 | Mesorhizobium loti |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.2 | - |
- |
Mesorhizobium loti |
9.2 | - |
50 mM CHES-NaOH buffer | Mesorhizobium loti |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.6 | 10 | pH 8.6: 66% of maximal activity, pH 10.0: 57% of maximal activity, 50 mM CHES-NaOH buffer | Mesorhizobium loti |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mesorhizobium loti |