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Literature summary for 1.1.1.107 extracted from

  • Trongpanich, Y.; Abe, K.; Kaneda, Y.; Morita, T.; Yagi, T.
    Purification and characterization of pyridoxal 4-dehydrogenase from Aureobacterium luteolum (2002), Biosci. Biotechnol. Biochem., 66, 543-548.
    View publication on PubMed
Search for more literature for 1.1.1.107

Inhibitors

Inhibitors Comment Organism Structure
8-hydroxyquinoline 1 mM, 78% inhibition Microbacterium luteolum
o-phenanthroline 1 mM, 15% inhibition Microbacterium luteolum
pyridoxal substrate inhibition, 90% inhibition at 2 mM Microbacterium luteolum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.133
-
 NAD+ 30°C, pH 8.0 Microbacterium luteolum
0.473
-
 pyridoxal 30°C, pH 8.0 Microbacterium luteolum
1.41
-
 NADP+ 30°C, pH 8.0 Microbacterium luteolum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 2 * 38000, SDS-PAGE Microbacterium luteolum
80000
-
 gel filtration Microbacterium luteolum

Organism

Organism UniProt Comment Textmining
Microbacterium luteolum
-
 growth on pyridoxine as carbon and nitrogen source
-

Purification (Commentary)

Purification (Comment) Organism
-
 Microbacterium luteolum

Storage Stability

Storage Stability Organism
4°C, 1% 2-mercaptoethanol, 24 h, no loss of activity, 2-mercaptoethanol is essential for stability Microbacterium luteolum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrate: pyridoxal-5’-phosphate, pyridoxamine, pyridoxine, 2-nitrobenzaldehyde, 3-nitrobenzaldehyde, 4-nitrobenzaldehyde, o-phthalaldehyde, 5-nitrosalicylaldehyde, 2-carboxybenzaldehyde Microbacterium luteolum ?
-
 ?
pyridine-4-aldehyde + NAD+ 0.9% of the activity against pyridoxal Microbacterium luteolum 4-pyridoxolactone + NADH + H+
-
 ?
pyridoxal + NAD+
-
 Microbacterium luteolum 4-pyridoxolactone + NADH + H+
-
 ?
pyridoxal + NADP+
-
 Microbacterium luteolum 4-pyridoxolactone + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 38000, SDS-PAGE Microbacterium luteolum
More N-terminal amino acid analysis Microbacterium luteolum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Microbacterium luteolum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 10 min, no residual activity Microbacterium luteolum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5 sodium phosphate buffer Microbacterium luteolum
8.5
-
 MOPS buffer Microbacterium luteolum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Microbacterium luteolum
NADP+
-
 Microbacterium luteolum