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Literature summary for 1.1.1.106 extracted from

  • Goodhue, C.T.; Snell, E.E.
    The bacterial degradation of pantothenic acid. II. Enzymatic formation of aldopantoic acid (1966), Biochemistry, 5, 403-408.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
D-pantoate at pH 10.0 Pseudomonas sp. P2
0.5
-
ketopantoate
-
Pseudomonas sp. P2

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-pantoate + NAD+ Pseudomonas sp. P2
-
(R)-4-dehydropantoate + NADH
-
r

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. P2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
50°C, protamine sulfate, calcium phosphate gel, ammonium sulfate, 50°C Pseudomonas sp. P2

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6
-
-
Pseudomonas sp. P2

Storage Stability

Storage Stability Organism
-20°C, 2 mM potassium phosphate, pH 7.0, 0.01% EDTA, 1 mM mercaptoethanol, several months, no loss of activity Pseudomonas sp. P2

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-pantoate + NAD+
-
Pseudomonas sp. P2 (R)-4-dehydropantoate + NADH
-
r
2-oxo-pantoate + NAD+
-
Pseudomonas sp. P2 2-oxo-4-dehydropantoate
-
?

pH Range

pH Minimum pH Maximum Comment Organism
7 10 linear activity increase Pseudomonas sp. P2

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas sp. P2