Cloned (Comment) | Organism |
---|---|
cloning of Rdh7 | Rattus norvegicus |
expression of His6-tagged RDH10 in Spodoptera frugiperda Sf9 insect cell membranes | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
L3R/L5R/R16Q/R19Q/R21Q | RDH1 mutant, deleting the positive charges from the C-terminal end of the leader, and inserting two arginine residues near the N-terminus of the signaling sequence causes 95% inversion from cytoplasmic to luminal, the mutant faces the lumen | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | N-ethylmaleimide does not affect the catalytic action of RDH1, but interferes with its approach to holo-CRBP1, interrupting retinol transfer. Modifying RDH with N-ethylmaleimide inhibited holo-CRBP1-supported generation of retinal by 90% | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | possible Michaelis-Menten relationship between RDH10 and holo-CRBP1 | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
cytosol | - |
Rattus norvegicus | 5829 | - |
endoplasmic reticulum membrane | cytoplasmic or lumenal facing, RDH1. An N-terminal signaling sequence of 22 residues anchors RDH1 in the endoplasmic reticulum projecting into the cytoplasm | Mus musculus | 5789 | - |
microsome | 80-94% of all enzyme activity | Mus musculus | - |
- |
microsome | 80-94% of all enzyme activity | Homo sapiens | - |
- |
microsome | 80-94% of all enzyme activity | Rattus norvegicus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
all-trans-retinol-[cellular-retinol-binding-protein] + NAD+ | Mus musculus | - |
all-trans-retinal-[cellular-retinol-binding-protein] + NADH + H+ | - |
ir | |
all-trans-retinol-[cellular-retinol-binding-protein] + NAD+ | Homo sapiens | - |
all-trans-retinal-[cellular-retinol-binding-protein] + NADH + H+ | - |
ir | |
all-trans-retinol-[cellular-retinol-binding-protein] + NAD+ | Rattus norvegicus | - |
all-trans-retinal-[cellular-retinol-binding-protein] + NADH + H+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
RDH1 activity during purification is maintained only with phosphotidylcholine | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
all-trans-retinol-[cellular-retinol-binding-protein] + NAD+ | - |
Mus musculus | all-trans-retinal-[cellular-retinol-binding-protein] + NADH + H+ | - |
ir | |
all-trans-retinol-[cellular-retinol-binding-protein] + NAD+ | - |
Homo sapiens | all-trans-retinal-[cellular-retinol-binding-protein] + NADH + H+ | - |
ir | |
all-trans-retinol-[cellular-retinol-binding-protein] + NAD+ | - |
Rattus norvegicus | all-trans-retinal-[cellular-retinol-binding-protein] + NADH + H+ | - |
ir | |
all-trans-retinol-[cellular-retinol-binding-protein] + NAD+ | RDH interact with/or recognize holo-CRBP1 | Homo sapiens | all-trans-retinal-[cellular-retinol-binding-protein] + NADH + H+ | - |
ir | |
all-trans-retinol-[cellular-retinol-binding-protein] + NAD+ | RDH interact with/or recognize holo-CRBP1 | Rattus norvegicus | all-trans-retinal-[cellular-retinol-binding-protein] + NADH + H+ | - |
ir | |
all-trans-retinol-[cellular-retinol-binding-protein] + NAD+ | RDH interact with/or recognize holo-CRBP1, except for RDH10 that does not access holo-CRBP1 as substrate | Mus musculus | all-trans-retinal-[cellular-retinol-binding-protein] + NADH + H+ | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
DHRS9 | - |
Mus musculus |
DHRS9 | - |
Homo sapiens |
DHRS9 | - |
Rattus norvegicus |
Rdh1 | - |
Mus musculus |
RDH10 | - |
Mus musculus |
RDH10 | - |
Homo sapiens |
RDH10 | - |
Rattus norvegicus |
RDH16 | - |
Homo sapiens |
RDH2 | - |
Rattus norvegicus |
RDH7 | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mus musculus | |
NAD+ | - |
Homo sapiens | |
NAD+ | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
evolution | RDH1, RDH10 and DHRS9 are microsomal members of the SDR gene family | Mus musculus |
malfunction | deleting the positive charges from the C-terminal end of the leader, and inserting two arginine residues near the N-terminus of the signaling sequence causes 95% inversion from cytoplasmic to luminal: i.e. the mutant L3R/L5R/R16Q/R19Q/R21Q faces the lumen | Mus musculus |
metabolism | the enzyme catalyzes the first step in all-trans-retinal biosynthesis, modeling of all-trans-retinal homeostasis, overview | Mus musculus |
metabolism | the enzyme catalyzes the first step in all-trans-retinal biosynthesis, modeling of all-trans-retinal homeostasis, overview | Homo sapiens |
metabolism | the enzyme catalyzes the first step in all-trans-retinal biosynthesis, modeling of all-trans-retinal homeostasis, overview | Rattus norvegicus |
additional information | N-ethylmaleimide does not affect the catalytic action of RDH1, but interferes with its approach to holo-CRBP1, interrupting retinol transfer. The membrane context of RDH affects function | Mus musculus |
physiological function | RDH1 contributes to a reconstituted pathway of all-trans-retinal biosynthesis, when expressed in intact cells with each of the three retinal dehydrogenases | Mus musculus |