Literature summary for 1.1.1.104 extracted from

Smith, T.E.; Mitoma, C.
Partial purification and some properties of 4-ketoproline reductase (1962), J. Biol. Chem., 237, 1177-1180.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.6	-	4-Oxoproline	-	Oryctolagus cuniculus
0.84	-	NADH	-	Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4-oxo-proline + NADH	Oryctolagus cuniculus	-	4-hydroxy-L-proline + NAD+	-	ir

Organism

Organism	UniProt	Comment	Textmining
Oryctolagus cuniculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate, negative alumina gel, positive alumina gel	Oryctolagus cuniculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
kidney	-	Oryctolagus cuniculus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
5.21	-	-	Oryctolagus cuniculus

Storage Stability

Storage Stability	Organism
-10°C, 160 mM phosphate buffer, pH 6.0, 3 months, no loss of activity	Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-oxo-proline + NADH	-	Oryctolagus cuniculus	4-hydroxy-L-proline + NAD+	-	ir
4-oxoproline + NADH	-	Oryctolagus cuniculus	4-hydroxy-L-proline + NAD+	-	ir

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	6.5	decrease of activity below pH 6.0	Oryctolagus cuniculus

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	Oryctolagus cuniculus

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Release 2023.1 (January 2023)