Protein Variants | Comment | Organism |
---|---|---|
D180A | 228fold decrease in the catalytic efficiency | Thermoplasma volcanium |
G114A | mutation cause a 4fold increase in catalytic efficiency | Thermoplasma volcanium |
I113A | 6fold decrease in the catalytic efficiency | Thermoplasma volcanium |
S74A | 1886fold decrease in the catalytic efficiency | Thermoplasma volcanium |
T178A | 18fold decrease in the catalytic efficiency | Thermoplasma volcanium |
T179A | 60fold decrease in the catalytic efficiency | Thermoplasma volcanium |
W273a | 413fold decrease in the catalytic efficiency | Thermoplasma volcanium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00057 | - |
L-threonine | 25°C, pH 10, mutant enzyme G114A | Thermoplasma volcanium | |
0.0026 | - |
L-threonine | 25°C, pH 10, wild-type enzyme | Thermoplasma volcanium | |
0.0088 | - |
L-threonine | 25°C, pH 10, mutant enzyme I113A | Thermoplasma volcanium | |
0.011 | - |
L-threonine | 25°C, pH 10, mutant enzyme T178A | Thermoplasma volcanium | |
0.043 | - |
L-threonine | 25°C, pH 10, mutant enzyme D180A | Thermoplasma volcanium | |
0.061 | - |
L-serine | 25°C, pH 10, wild-type enzyme | Thermoplasma volcanium | |
0.18 | - |
L-serine | 25°C, pH 10, mutant enzyme G114A | Thermoplasma volcanium | |
0.36 | - |
(R)-(-)-1-amino-2-propanol | 25°C, pH 10, mutant enzyme G114A | Thermoplasma volcanium | |
1.6 | - |
L-serine | 25°C, pH 10, mutant enzyme T178A | Thermoplasma volcanium | |
1.8 | - |
L-serine | 25°C, pH 10, mutant enzyme I113A | Thermoplasma volcanium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine + NAD+ | Thermoplasma volcanium | - |
L-2-amino-3-oxobutanoate + NADH + H+ | - |
? | |
L-threonine + NAD+ | Thermoplasma volcanium ATCC 51530 | - |
L-2-amino-3-oxobutanoate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoplasma volcanium | Q97BK3 | - |
- |
Thermoplasma volcanium ATCC 51530 | Q97BK3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-(-)-1-amino-2-propanol + NAD+ | activity with mutant enzyme G114A. No activity with wild-type enzyme | Thermoplasma volcanium | ? | - |
? | |
(R)-(-)-1-amino-2-propanol + NAD+ | activity with mutant enzyme G114A. No activity with wild-type enzyme | Thermoplasma volcanium ATCC 51530 | ? | - |
? | |
L-serine + NAD+ | - |
Thermoplasma volcanium | L-2-amino-3-oxopropionate + NADH + H+ | - |
? | |
L-serine + NAD+ | - |
Thermoplasma volcanium ATCC 51530 | L-2-amino-3-oxopropionate + NADH + H+ | - |
? | |
L-threonine + NAD+ | - |
Thermoplasma volcanium | L-2-amino-3-oxobutanoate + NADH + H+ | - |
? | |
L-threonine + NAD+ | - |
Thermoplasma volcanium ATCC 51530 | L-2-amino-3-oxobutanoate + NADH + H+ | - |
? | |
L-threoninol + NAD+ | - |
Thermoplasma volcanium | ? | - |
? | |
L-threoninol + NAD+ | - |
Thermoplasma volcanium ATCC 51530 | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0085 | - |
(R)-(-)-1-amino-2-propanol | 25°C, pH 10, mutant enzyme G114A | Thermoplasma volcanium | |
0.01 | - |
L-serine | 25°C, pH 10, wild-type enzyme | Thermoplasma volcanium | |
0.013 | - |
L-serine | 25°C, pH 10, mutant enzyme G114A | Thermoplasma volcanium | |
0.014 | - |
L-serine | 25°C, pH 10, mutant enzyme T178A | Thermoplasma volcanium | |
0.026 | - |
L-serine | 25°C, pH 10, mutant enzyme I113A | Thermoplasma volcanium | |
0.37 | - |
L-threonine | 25°C, pH 10, mutant enzyme D180A | Thermoplasma volcanium | |
0.73 | - |
L-threonine | 25°C, pH 10, mutant enzyme I113A | Thermoplasma volcanium | |
1.6 | - |
L-threonine | 25°C, pH 10, mutant enzyme G114A | Thermoplasma volcanium | |
1.7 | - |
L-threonine | 25°C, pH 10, wild-type enzyme | Thermoplasma volcanium |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000087 | - |
L-threoninol | 25°C, pH 10, wild-type enzyme | Thermoplasma volcanium | |
0.0000035 | - |
(R)-(-)-1-amino-2-propanol | 25°C, pH 10, mutant enzyme G114A | Thermoplasma volcanium | |
0.0000095 | - |
L-serine | 25°C, pH 10, mutant enzyme T178A | Thermoplasma volcanium | |
0.000023 | - |
L-threoninol | 25°C, pH 10, wild-type enzyme | Thermoplasma volcanium | |
0.000055 | - |
L-serine | 25°C, pH 10, mutant enzyme I113A | Thermoplasma volcanium | |
0.00017 | - |
L-serine | 25°C, pH 10, wild-type enzyme | Thermoplasma volcanium | |
0.00035 | - |
L-threonine | 25°C, pH 10, mutant enzyme T178A | Thermoplasma volcanium | |
0.00035 | - |
L-threonine | 25°C, pH 10, mutant enzyme W273A | Thermoplasma volcanium | |
0.00054 | - |
L-serine | 25°C, pH 10, mutant enzyme G114A | Thermoplasma volcanium | |
0.0016 | - |
L-threonine | 25°C, pH 10, mutant enzyme T179A | Thermoplasma volcanium | |
0.0029 | - |
L-threonine | 25°C, pH 10, mutant enzyme S74A | Thermoplasma volcanium | |
0.011 | - |
L-threonine | 25°C, pH 10, mutant enzyme D180A | Thermoplasma volcanium | |
0.11 | - |
L-threonine | 25°C, pH 10, mutant enzyme I113A | Thermoplasma volcanium | |
0.66 | - |
L-threonine | 25°C, pH 10, wild-type enzyme | Thermoplasma volcanium | |
2.7 | - |
L-threonine | 25°C, pH 10, mutant enzyme G114A | Thermoplasma volcanium |