Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.103 extracted from

  • He, C.; Huang, X.; Liu, Y.; Li, F.; Yang, Y.; Tao, H.; Han, C.; Zhao, C.; Xiao, Y.; Shi, Y.
    Structural insights on mouse L-threonine dehydrogenase: A regulatory role of Arg180 in catalysis (2015), J. Struct. Biol., 192, 510-518.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
apo form, in complex with NAD+ and glycerol, or with only NAD+, sitting drop vapor diffusion method, using 0.1 M HEPES (pH 7.0), 10% (w/v) PEG 4000, 10% (v/v) 2-propanol, or 0.2 M NaCl, 0.1 M HEPES (pH 7.5), 25% (w/v) PEG3350 Mus musculus

Protein Variants

Protein Variants Comment Organism
M333A inactive Mus musculus
M333E the mutation affects the substrate's affinity for the enzyme Mus musculus
R180K the mutation has little effect on NAD+ binding affinity, whereas affects the substrate's affinity for the enzyme Mus musculus
S133A inactive Mus musculus
T237A inactive Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.67
-
NAD+ wild type enzyme, at pH 7.5 and 37°C Mus musculus
2.06
-
NAD+ mutant enzyme R180K, at pH 7.5 and 37°C Mus musculus
72.4
-
L-threonine wild type enzyme, at pH 7.5 and 37°C Mus musculus
151.9
-
L-threonine mutant enzyme M333E, at pH 7.5 and 37°C Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Mus musculus 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-threonine + NAD+ Mus musculus
-
(2S)-2-amino-3-oxobutanoate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus Q8K3F7
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-chelating column chromatography and Superdex 200 gel filtration Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-threonine + NAD+
-
Mus musculus (2S)-2-amino-3-oxobutanoate + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
L-threonine dehydrogenase
-
Mus musculus
TDH
-
Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
46.7
-
L-threonine mutant enzyme M333E, at pH 7.5 and 37°C Mus musculus
47.3
-
L-threonine wild type enzyme, at pH 7.5 and 37°C Mus musculus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.003
-
L-threonine mutant enzyme R180K, at pH 7.5 and 37°C Mus musculus
0.004
-
L-threonine mutant enzyme S133A, at pH 7.5 and 37°C Mus musculus
0.004
-
L-threonine mutant enzyme T237A, at pH 7.5 and 37°C Mus musculus
0.006
-
L-threonine mutant enzyme M333A, at pH 7.5 and 37°C Mus musculus
0.3
-
L-threonine mutant enzyme M333E, at pH 7.5 and 37°C Mus musculus
0.7
-
L-threonine wild type enzyme, at pH 7.5 and 37°C Mus musculus