Cloned (Comment) | Organism |
---|---|
gene tdh, functional expression in Escherichia coli | Pyrococcus furiosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | complete inhibition, fully reversible by Zn2+ or Co2+ | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.055 | - |
NAD+ | pH 7.0, 70°C, recombinant enzyme | Pyrococcus furiosus | |
1.5 | - |
L-threonine | pH 7.0, 70°C, recombinant enzyme | Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates | Pyrococcus furiosus | |
Zn2+ | 2 Zn2+ per enzyme subunit, binding involves residues G168, G175, G180, and G212, overview | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37823 | - |
4 * 40000, recombinant enzyme, SDS-PAGE, 4 * 37823, sequence calculation | Pyrococcus furiosus |
40000 | - |
4 * 40000, recombinant enzyme, SDS-PAGE, 4 * 37823, sequence calculation | Pyrococcus furiosus |
155000 | - |
about, recombinant enzyme, gel filtration | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine + NAD+ | Pyrococcus furiosus | - |
(2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
gene tdh | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 48fold from Escherichia coli to homogeneity by heat treatment and anion exchange chromatography | Pyrococcus furiosus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-threonine + NAD+ = L-2-amino-3-oxobutanoate + NADH + H+ | His94 is an active site residue, substrate binding involved residues Gly66, Gly71, Gly77, and Val80 | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butane-2,3-diol + NAD+ | - |
Pyrococcus furiosus | ? + NADH | - |
? | |
D-threonine + NAD+ | - |
Pyrococcus furiosus | D-2-amino-3-oxobutyrate + NADH | - |
? | |
L-serine + NAD+ | - |
Pyrococcus furiosus | ? + NADH | - |
? | |
L-threonine + NAD+ | - |
Pyrococcus furiosus | (2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? | |
L-threonine + NAD+ | substrate binding involved residues G66, G71, G77, and V80, H94 is an active site residue | Pyrococcus furiosus | (2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? | |
additional information | broad substrate specificity, determinants, overview | Pyrococcus furiosus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 40000, recombinant enzyme, SDS-PAGE, 4 * 37823, sequence calculation | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
L-threonine dehydrogenase | - |
Pyrococcus furiosus |
TDH | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Pyrococcus furiosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pyrococcus furiosus |