Cloned (Comment) | Organism |
---|---|
- |
Cytophaga sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine-5'-diphosphoribose | - |
Cytophaga sp. | |
CuCl2 | 1 mM, 100% inhibition | Cytophaga sp. | |
HgCl2 | 10 mM, 100% inhibition | Cytophaga sp. | |
monoiodoacetate | 10 mM, 100% inhibition | Cytophaga sp. | |
N-ethylmaleimide | 10 mM, 48% inhibition | Cytophaga sp. | |
NADH | competitive to NAD+, noncompetitive to L-threonine | Cytophaga sp. | |
p-chloromercuribenzoic acid | 10 mM, 44% inhibition | Cytophaga sp. | |
pyruvate | competitive to L-threonine | Cytophaga sp. | |
ZnCl2 | 1 mM, 72% inhibition | Cytophaga sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.56 | - |
L-threonine | pH 9.0, 20°C | Cytophaga sp. | |
3.81 | - |
L-threonine | pH 9.0, 10°C | Cytophaga sp. | |
5.96 | - |
L-threonine | pH 9.0, 30°C | Cytophaga sp. | |
11.2 | - |
L-threonine | pH 9.0, 40°C | Cytophaga sp. | |
19.5 | - |
L-threonine | pH 9.0, 50°C | Cytophaga sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
4 * 35000, SDS-PAGE | Cytophaga sp. |
139000 | - |
gel filtration | Cytophaga sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cytophaga sp. | Q8KZM4 | - |
- |
Cytophaga sp. KUC-1 | Q8KZM4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Cytophaga sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-threonine + NAD+ = L-2-amino-3-oxobutanoate + NADH + H+ | ordered bi-bi mechanism, NAD+ binds prior to L-threonine | Cytophaga sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
91.4 | - |
pH 9.0, 10°C | Cytophaga sp. |
156 | - |
pH 9.0, 20°C | Cytophaga sp. |
284 | - |
pH 9.0, 30°C | Cytophaga sp. |
611 | - |
pH 9.0, 50°C | Cytophaga sp. |
722 | - |
pH 9.0, 40°C | Cytophaga sp. |
Storage Stability | Organism |
---|---|
-20°C, 10 mM potassium phosphate, pH 7.0, 30% sucrose, 0.1 mM NAD+, 5 mM L-threonine, several months, no loss of activity | Cytophaga sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-threo-3-hydroxynorvaline + NAD+ | 31% the rate of L-threonine | Cytophaga sp. | ? + NADH | - |
? | |
DL-threo-3-hydroxynorvaline + NAD+ | 31% the rate of L-threonine | Cytophaga sp. KUC-1 | ? + NADH | - |
? | |
L-threonine + 3-acetyl-pyridine adenine dinucleotide | 60.6% the rate of NAD+ | Cytophaga sp. | L-2-amino-3-oxobutanoate + ? | - |
? | |
L-threonine + 3-acetyl-pyridine adenine dinucleotide | 60.6% the rate of NAD+ | Cytophaga sp. KUC-1 | L-2-amino-3-oxobutanoate + ? | - |
? | |
L-threonine + 3-pyridinealdehyde adenine dinucleotide | 7.2% the rate of NAD+ | Cytophaga sp. | L-2-amino-3-oxobutanoate + ? | - |
? | |
L-threonine + 3-pyridinealdehyde adenine dinucleotide | 7.2% the rate of NAD+ | Cytophaga sp. KUC-1 | L-2-amino-3-oxobutanoate + ? | - |
? | |
L-threonine + NAD+ | - |
Cytophaga sp. | (2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? | |
L-threonine + NAD+ | - |
Cytophaga sp. KUC-1 | (2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? | |
L-threonine + nicotinamide guanine dinucleotide | 5.1% the rate of NAD+ | Cytophaga sp. | L-2-amino-3-oxobutanoate + ? | - |
? | |
L-threonine + thionicotinamide-NAD+ | 5.1% the rate of NAD+ | Cytophaga sp. | L-2-amino-3-oxobutanoate + ? | - |
? | |
additional information | specific for L-form of threonine, no substrate: NADP, nicotinic acid adenine dinucleotide, alpha-NAD, nicotinamide hypoxanthine dinucleotide | Cytophaga sp. | ? | - |
? | |
additional information | specific for L-form of threonine, no substrate: NADP, nicotinic acid adenine dinucleotide, alpha-NAD, nicotinamide hypoxanthine dinucleotide | Cytophaga sp. KUC-1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 35000, SDS-PAGE | Cytophaga sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Cytophaga sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 30 | 60 min, more than 90% residual activity | Cytophaga sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
half-life 160 min | Cytophaga sp. |
45 | - |
half-life 14 min | Cytophaga sp. |
50 | - |
rapid inactivation | Cytophaga sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Cytophaga sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
74% of maximum activity | Cytophaga sp. |
10.5 | - |
31% of maximum activity | Cytophaga sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
3-acetyl-pyridine adenine dinucleotide | - |
Cytophaga sp. | |
nicotinamide guanine dinucleotide | - |
Cytophaga sp. | |
thionicotinamide-NAD+ | - |
Cytophaga sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.37 | - |
adenosine-5'-diphosphoribose | substrate NAD+, pH 9.0, 30°C | Cytophaga sp. | |
1.42 | - |
adenosine-5'-diphosphoribose | substrate L-threonine, pH 9.0, 30°C | Cytophaga sp. | |
23.2 | - |
pyruvate | substrate L-threonine, pH 9.0, 30°C | Cytophaga sp. | |
50.7 | - |
pyruvate | substrate NAD+, pH 9.0, 30°C | Cytophaga sp. |