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Literature summary for 1.1.1.103 extracted from

  • McGilvray, D.; Morris, J.G.
    L-Threonine dehydrogenase (Arthrobacter) (1971), Methods Enzymol., 17B, 580-584.
No PubMed abstract available

General Stability

General Stability Organism
most stable in Tris-HCl, pH 8, purified preparations in phosphate buffers lose their activity overnight Arthrobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Arthrobacter sp.
EDTA
-
Arthrobacter sp.
Hg2+
-
Arthrobacter sp.
p-mercuribenzoate
-
Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
NAD+ at pH 7.5 Arthrobacter sp.
10.5
-
L-threonine at pH 7.5 Arthrobacter sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ restores enzyme activity after EDTA treatment Arthrobacter sp.
Mn2+ restores enzyme activity after EDTA treatment Arthrobacter sp.
Zn2+ restores enzyme activity after EDTA treatment Arthrobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-threonine + NAD+ Arthrobacter sp.
-
(2S)-2-amino-3-oxobutanoate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
protamine sulfate, Sephadex G-200, DEAE-cellulose Arthrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.05
-
-
Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-threonine + NAD+
-
Arthrobacter sp. (2S)-2-amino-3-oxobutanoate + NADH + H+
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 8.9
-
Arthrobacter sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Arthrobacter sp.