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Literature summary for 1.1.1.103 extracted from

  • Aoyama, Y.; Motokawa, Y.
    L-Threonine dehydrogenase of chicken liver (1981), J. Biol. Chem., 256, 12367-12373.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
adenosine-5'-diphosphoribose competive inhibition vs. NAD+, noncompetitive inhibition vs. L-threonine Gallus gallus
Cd2+ 0.05 and 1.0 mM, 90% inhibition Gallus gallus
Cu2+ 0.05 and 1.0 mM, 90% inhibition Gallus gallus
Mn2+ 1.0 mM, 40-50% inhibition Gallus gallus
NADH competitive inhibition vs. NAD+, noncompetitive vs. L-threonine Gallus gallus
Ni2+ 1 mM, 40-50% inhibition Gallus gallus
Zn2+ 0.05 and 1.0 mM, 90% inhibition Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.98
-
NAD+
-
Gallus gallus
8.4
-
L-threonine
-
Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
gel filtration Gallus gallus
88000
-
density gradient Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-threonine + NAD+ Gallus gallus
-
(2S)-2-amino-3-oxobutanoate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose, ammonium sulfate, DEAE-cellulose, Blue dextran-Sepharose, Sephadex G-200, amylamine sepharose, Sephadex G-200 Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19
-
-
Gallus gallus

Storage Stability

Storage Stability Organism
-20°C, 14 mM 2-mercaptoethanol, 20% glycerol, at least 1 month, little loss of activity Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-threonine + NAD+
-
Gallus gallus (2S)-2-amino-3-oxobutanoate + NADH + H+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 88000, SDS-PAGE Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6 8.7 30% activity at pH 7.5, 80% activity at pH 9.8 Gallus gallus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Gallus gallus