Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine-5'-diphosphoribose | competive inhibition vs. NAD+, noncompetitive inhibition vs. L-threonine | Gallus gallus | |
Cd2+ | 0.05 and 1.0 mM, 90% inhibition | Gallus gallus | |
Cu2+ | 0.05 and 1.0 mM, 90% inhibition | Gallus gallus | |
Mn2+ | 1.0 mM, 40-50% inhibition | Gallus gallus | |
NADH | competitive inhibition vs. NAD+, noncompetitive vs. L-threonine | Gallus gallus | |
Ni2+ | 1 mM, 40-50% inhibition | Gallus gallus | |
Zn2+ | 0.05 and 1.0 mM, 90% inhibition | Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.98 | - |
NAD+ | - |
Gallus gallus | |
8.4 | - |
L-threonine | - |
Gallus gallus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
gel filtration | Gallus gallus |
88000 | - |
density gradient | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine + NAD+ | Gallus gallus | - |
(2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose, ammonium sulfate, DEAE-cellulose, Blue dextran-Sepharose, Sephadex G-200, amylamine sepharose, Sephadex G-200 | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19 | - |
- |
Gallus gallus |
Storage Stability | Organism |
---|---|
-20°C, 14 mM 2-mercaptoethanol, 20% glycerol, at least 1 month, little loss of activity | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine + NAD+ | - |
Gallus gallus | (2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 88000, SDS-PAGE | Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | 8.7 | 30% activity at pH 7.5, 80% activity at pH 9.8 | Gallus gallus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Gallus gallus |