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Literature summary for 1.1.1.103 extracted from

  • Ray, M.; Ray, S.
    L-Threonine dehydrogenase from goat liver (1985), J. Biol. Chem., 260, 5913-5918.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
methylglyoxal 1.0 mM, 42% inhibition, 2 mM, 63% inhibition, methylglyoxal binds at an allosteric site of the enzyme Capra hircus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
NAD+
-
Capra hircus
5.5
-
L-threonine
-
Capra hircus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
4 * 35000, SDS-PAGE Capra hircus
86000
-
gel filtration Capra hircus
89000
-
1 * 89000, SDS-PAGE Capra hircus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-threonine + NAD+ Capra hircus
-
(2S)-2-amino-3-oxobutanoate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Capra hircus
-
goat
-

Purification (Commentary)

Purification (Comment) Organism
acid precipitation, ammonium sulfate, Sephadex G-100, Sephadex G-200 Capra hircus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Capra hircus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.45
-
-
Capra hircus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-threonine + NAD+
-
Capra hircus (2S)-2-amino-3-oxobutanoate + NADH + H+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 89000, SDS-PAGE Capra hircus
tetramer 4 * 35000, SDS-PAGE Capra hircus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2 8.8
-
Capra hircus

pH Range

pH Minimum pH Maximum Comment Organism
7.4 9
-
Capra hircus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Capra hircus