Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methylglyoxal | 1.0 mM, 42% inhibition, 2 mM, 63% inhibition, methylglyoxal binds at an allosteric site of the enzyme | Capra hircus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
NAD+ | - |
Capra hircus | |
5.5 | - |
L-threonine | - |
Capra hircus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
4 * 35000, SDS-PAGE | Capra hircus |
86000 | - |
gel filtration | Capra hircus |
89000 | - |
1 * 89000, SDS-PAGE | Capra hircus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine + NAD+ | Capra hircus | - |
(2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Capra hircus | - |
goat | - |
Purification (Comment) | Organism |
---|---|
acid precipitation, ammonium sulfate, Sephadex G-100, Sephadex G-200 | Capra hircus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Capra hircus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.45 | - |
- |
Capra hircus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine + NAD+ | - |
Capra hircus | (2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 89000, SDS-PAGE | Capra hircus |
tetramer | 4 * 35000, SDS-PAGE | Capra hircus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | 8.8 | - |
Capra hircus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.4 | 9 | - |
Capra hircus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Capra hircus |