Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 0.064 mM, activity is completely blocked | Escherichia coli K-12 | |
Be2+ | 3.2 mM, 20-50% inhibition | Escherichia coli K-12 | |
Cu2+ | 3.2 mM, 20-50% inhibition | Escherichia coli K-12 | |
Hg2+ | 0.064 mM, activity is completely blocked | Escherichia coli K-12 | |
Ni2+ | 3.2 mM, 20-50% inhibition | Escherichia coli K-12 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
NAD+ | demetallized enzyme | Escherichia coli K-12 | |
0.6 | - |
NAD+ | enzyme saturated with 0.25 mM Mn2+ | Escherichia coli K-12 | |
1.1 | - |
L-threonine | demetallized enzyme | Escherichia coli K-12 | |
221 | - |
L-threonine | enzyme saturated with 0.25 mM Mn2+ | Escherichia coli K-12 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | 0.064 or 3.2 mM, 6fold increase of L-threonine dehydrogenase activity | Escherichia coli K-12 | |
Mn2+ | 0.064 or 3.2 mM, 4fold increase of L-threonine dehydrogenase activity, activation is dependent on the presence of a reduced thiol in all enzyme stock solutions and assay buffers, binding of 0.86 mol of Mn2+ per mol of enzyme subunit | Escherichia coli K-12 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine + NAD+ | Escherichia coli K-12 | - |
(2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli K-12 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli K-12 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8 | - |
fully demetalized enzyme | Escherichia coli K-12 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine + NAD+ | - |
Escherichia coli K-12 | (2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli K-12 |