Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.103 extracted from

  • Craig, P.A.; Dekker, E.E.
    L-Threonine dehydrogenase from Escherichia coli K-12: thiol-dependent activation by Mn2+ (1986), Biochemistry, 25, 1870-1876.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 0.064 mM, activity is completely blocked Escherichia coli K-12
Be2+ 3.2 mM, 20-50% inhibition Escherichia coli K-12
Cu2+ 3.2 mM, 20-50% inhibition Escherichia coli K-12
Hg2+ 0.064 mM, activity is completely blocked Escherichia coli K-12
Ni2+ 3.2 mM, 20-50% inhibition Escherichia coli K-12

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
NAD+ demetallized enzyme Escherichia coli K-12
0.6
-
NAD+ enzyme saturated with 0.25 mM Mn2+ Escherichia coli K-12
1.1
-
L-threonine demetallized enzyme Escherichia coli K-12
221
-
L-threonine enzyme saturated with 0.25 mM Mn2+ Escherichia coli K-12

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ 0.064 or 3.2 mM, 6fold increase of L-threonine dehydrogenase activity Escherichia coli K-12
Mn2+ 0.064 or 3.2 mM, 4fold increase of L-threonine dehydrogenase activity, activation is dependent on the presence of a reduced thiol in all enzyme stock solutions and assay buffers, binding of 0.86 mol of Mn2+ per mol of enzyme subunit Escherichia coli K-12

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-threonine + NAD+ Escherichia coli K-12
-
(2S)-2-amino-3-oxobutanoate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli K-12
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli K-12

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8
-
fully demetalized enzyme Escherichia coli K-12

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-threonine + NAD+
-
Escherichia coli K-12 (2S)-2-amino-3-oxobutanoate + NADH + H+
-
?

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Escherichia coli K-12