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Literature summary for 1.1.1.101 extracted from
- Acyl/alkyldihydroxyacetone phosphate reductase from guinea pig liver peroxisomes (1992), Methods Enzymol., 209, 402-407.
General Stability
| General Stability | Organism |
|---|---|
| unstable in the absence of NADPH | Cavia porcellus |
| unstable on freezing and thawing | Cavia porcellus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
NADPH | cosubstrate palmitoydihydroxyacetone phosphate | Cavia porcellus |  |
| 0.02 | - |
NADPH | cosubstrate hexadecyldihydroxyacetone phosphate | Cavia porcellus | |
| 0.021 | - |
Hexadecyldihydroxyacetone phosphate | cosubstrate NADPH | Cavia porcellus | |
| 1.7 | - |
NADH | - |
Cavia porcellus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
1 * 60000, SDS-PAGE | Cavia porcellus |
| 75000 | - |
liver peroxisomes, gel filtration in the presence of octylglucoside | Cavia porcellus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography on NADPH-agarose gel matrix | Cavia porcellus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Cavia porcellus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 62.5 | - |
- |
Cavia porcellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyldihydroxyacetone phosphate + NADPH | - |
Cavia porcellus | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| alkyldihydroxyacetone phosphate + NADPH + H+ | - |
Cavia porcellus | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| hexadecyl dihydroxyacetone phosphate + NADH + H+ | - |
Cavia porcellus | 1-hexadecyl-sn-glycerol 3-phosphate + NAD+ | - |
r | |
| hexadecyldihydroxyacetone phosphate + NADPH | - |
Cavia porcellus | 1-hexadecyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| palmitoylglycerone phosphate + NADPH | i.e. palmitoyldihydroxyacetone phosphate | Cavia porcellus | 1-palmitoylglycerol 3-phosphate + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 60000, SDS-PAGE | Cavia porcellus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
stable for 4-6 weeks in the presence of octylglucoside and NADPH | Cavia porcellus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | broad optimum | Cavia porcellus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | can substitute for NADPH with very low activity and affinity | Cavia porcellus | |
| NADPH | hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates | Cavia porcellus | |
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Release 2023.1 (January 2023)
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