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Literature summary for 1.1.1.101 extracted from
- Subcellular distribution and properties of acyl/alkyl dihydroxyacetone phosphate reductase in rodent livers (1986), Arch. Biochem. Biophys., 245, 523-530.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Clofibrate | activation | Mus musculus |  |
General Stability
| General Stability | Organism |
|---|---|
| NADPH enhances thermal stability | Cavia porcellus |
| stable toward high concentrations of N-ethylmaleimide or DTNB | Cavia porcellus |
| thiols stabilize solubilized enzyme | Cavia porcellus |
| Triton X-100, stable to solubilization in the presence of high salt concentrations | Cavia porcellus |
| trypsin inactivates | Cavia porcellus |
| trypsin inactivates | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alkyldihydroxyacetone phosphate | at high concentrations | Cavia porcellus | |
| additional information | DTNB and thiols; stable to N-ethylmaleimide | Cavia porcellus | |
| NADP+ | competitive to NADPH | Cavia porcellus | |
| palmitoyl-CoA | - |
Cavia porcellus | |
| Trypsin | inactivation | Cavia porcellus | |
| Trypsin | inactivation | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
NADPH | - |
Rattus norvegicus | |
| 0.01 | - |
NADPH | Km-value below: hexadecyldihydroxyacetone phosphate + NADPH, microsomal enzyme | Cavia porcellus | |
| 0.02 | - |
NADPH | cosubstrate palmitoyldihydroxyacetone phosphate, microsomal enzyme | Cavia porcellus | |
| 0.02 | - |
NADPH | ,cosubstrate hexadecyldihydroxyacetone phosphate, microsomal enzyme | Cavia porcellus | |
| 0.05 | - |
NADPH | hexadecyldihydroxyacetone phosphate, peroxisomal enzyme | Cavia porcellus | |
| 0.06 | - |
NADPH | cosubstrate palmitoyldihydroxyacetone phosphate, peroxisomal enzyme | Cavia porcellus | |
| 0.07 | - |
NADPH | in presence of NADP+, microsomal enzyme | Cavia porcellus | |
| 0.08 | - |
NADH | cosubstrate hexadecyldihydroxyacetone phosphate, microsomal enzyme, NADPH, cosubstrate hexadecyldihydroxyacetone phosphate, peroxisomal enzyme | Cavia porcellus | |
| 0.08 | - |
NADH | enzyme isolated from microsome | Cavia porcellus | |
| 0.13 | - |
NADPH | in presence of NADP+, peroxisomal enzyme | Cavia porcellus | |
| 0.8 | - |
NADH | cosubstrate hexadecyldihydroxyacetone phosphate, peroxisomal enzyme | Cavia porcellus | |
| 0.8 | - |
NADH | enzyme isolated from peroxisome | Cavia porcellus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyldihydroxyacetone phosphate + NADPH | Cavia porcellus | reaction in non-ether lipids biosynthesis | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| acyldihydroxyacetone phosphate + NADPH | Mus musculus | reaction in non-ether lipids biosynthesis | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| acyldihydroxyacetone phosphate + NADPH | Rattus norvegicus | reaction in non-ether lipids biosynthesis | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| alkyldihydroxyacetone phosphate + NADPH + H+ | Cavia porcellus | reaction in ether lipids biosynthesis | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| alkyldihydroxyacetone phosphate + NADPH + H+ | Mus musculus | reaction in ether lipids biosynthesis | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| alkyldihydroxyacetone phosphate + NADPH + H+ | Rattus norvegicus | reaction in ether lipids biosynthesis | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
- |
- |
| Mus musculus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, solubilization | Cavia porcellus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Cavia porcellus | - |
| liver | - |
Mus musculus | - |
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0186 | - |
light mitochondrial fraction | Mus musculus |
| 0.0296 | - |
light mitochondrial fraction, clofibrate-fed animals | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyldihydroxyacetone phosphate + NADPH | - |
Cavia porcellus | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| acyldihydroxyacetone phosphate + NADPH | - |
Mus musculus | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| acyldihydroxyacetone phosphate + NADPH | - |
Rattus norvegicus | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| acyldihydroxyacetone phosphate + NADPH | reaction in non-ether lipids biosynthesis | Cavia porcellus | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| acyldihydroxyacetone phosphate + NADPH | reaction in non-ether lipids biosynthesis | Mus musculus | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| acyldihydroxyacetone phosphate + NADPH | reaction in non-ether lipids biosynthesis | Rattus norvegicus | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| alkyldihydroxyacetone phosphate + NADPH + H+ | - |
Mus musculus | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| alkyldihydroxyacetone phosphate + NADPH + H+ | ether analog of acyl dihydroxyacetone phosphate | Cavia porcellus | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| alkyldihydroxyacetone phosphate + NADPH + H+ | ether analog of acyl dihydroxyacetone phosphate | Rattus norvegicus | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| alkyldihydroxyacetone phosphate + NADPH + H+ | reaction in ether lipids biosynthesis | Cavia porcellus | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| alkyldihydroxyacetone phosphate + NADPH + H+ | reaction in ether lipids biosynthesis | Mus musculus | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| alkyldihydroxyacetone phosphate + NADPH + H+ | reaction in ether lipids biosynthesis | Rattus norvegicus | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| hexadecyl dihydroxyacetone phosphate + NADH + H+ | - |
Cavia porcellus | 1-hexadecyl-sn-glycerol 3-phosphate + NAD+ | - |
r | |
| hexadecyldihydroxyacetone phosphate + NADPH | - |
Cavia porcellus | 1-hexadecyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| hexadecyldihydroxyacetone phosphate + NADPH | - |
Rattus norvegicus | 1-hexadecyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| palmitoylglycerone phosphate + NADPH | i.e. palmitoyldihydroxyacetone phosphate | Cavia porcellus | 1-palmitoylglycerol 3-phosphate + NADP+ | - |
? | |
| palmitoylglycerone phosphate + NADPH | i.e. palmitoyldihydroxyacetone phosphate | Rattus norvegicus | 1-palmitoylglycerol 3-phosphate + NADP+ | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
10% loss of activity after 15 min, NADPH enhances thermal stability | Cavia porcellus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Cavia porcellus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8.2 | about half-maximal activity at pH 5.5 and 8.2 | Cavia porcellus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Cavia porcellus | |
| NADH | - |
Rattus norvegicus | |
| NADPH | hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates | Cavia porcellus | |
| NADPH | hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates | Mus musculus | |
| NADPH | hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates | Rattus norvegicus | |
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