Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.100 extracted from

  • Hou, J.; Zheng, H.; Chruszcz, M.; Zimmerman, M.D.; Shumilin, I.A.; Osinski, T.; Demas, M.; Grimshaw, S.; Minor, W.
    Dissecting the structural elements for the activation of beta-ketoacyl-(acyl carrier protein) reductase from Vibrio cholerae (2015), J. Bacteriol., 198, 463-476.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL cells Vibrio cholerae serotype O1

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 2.0-2.7 M ammonium sulfate and 0.1 M Tris (pH 9.0) Vibrio cholerae serotype O1

Protein Variants

Protein Variants Comment Organism
G141A the mutant shows less than 5% of wild type activity Vibrio cholerae serotype O1
G92A the mutant shows less than 60% of wild type activity Vibrio cholerae serotype O1
G92D the mutant shows less than 5% of wild type activity Vibrio cholerae serotype O1
Q152A the mutant shows less than 10% of wild type activity Vibrio cholerae serotype O1
Y155F the mutant shows less than 3% of wild type activity Vibrio cholerae serotype O1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
-
Vibrio cholerae serotype O1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-oxoacyl-[acyl-carrier protein] + NADPH + H+ Vibrio cholerae serotype O1
-
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Vibrio cholerae serotype O1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography and Superdex 200 pg gel filtration Vibrio cholerae serotype O1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-oxoacyl-[acyl-carrier protein] + NADPH + H+
-
Vibrio cholerae serotype O1 (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
?
acetoacetyl-CoA + NADPH + H+
-
Vibrio cholerae serotype O1 ?
-
?

Subunits

Subunits Comment Organism
homodimer or homotetramer 2 or 4 * 25000, SDS-PAGE Vibrio cholerae serotype O1

Synonyms

Synonyms Comment Organism
beta-ketoacyl-(acyl carrier protein) reductase
-
Vibrio cholerae serotype O1
FabG
-
Vibrio cholerae serotype O1

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Vibrio cholerae serotype O1