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Literature summary for 1.1.1.100 extracted from
- Comparative effect of overexpressed phaJ and fabG genes supplementing (R)-3-hydroxyalkanoate monomer units on biosynthesis of mcl-polyhydroxyalkanoate in Pseudomonas putida KCTC1639 (2008), J. Biosci. Bioeng., 106, 95-98.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Recombinant Escherichia coli (strain DH5alpha) co-expresses the fabG gene. The fabG gene is PCR-cloned using primers derived from the up and down-stream regions of the Pseudomonas putida KT2440 genome. | Pseudomonas putida |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25500 | - |
derived from amino acid sequence containing 246 amino acids | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ | Pseudomonas putida | beta-oxidation pathway | 3-oxoacyl-[acyl-carrier protein] + NADPH + H+ | - |
? |  |
| additional information | Pseudomonas putida | fabG overexpression tends to depress the biosynthesis of medium-chain-length polyhydroxyalkanoate due to the reversible conversion of (R)-3-hydroxyalkanoate monomer units into 3-ketoacyl-CoA | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | A5HIF6 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | strain KCTC1639 | Pseudomonas putida | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the intrinsic NADPH dependent 3-ketoacyl-CoA reductase activity in the transformant Pseudomonas putida with overexpressed fabG gene increased about 1.6-fold from 0.87 to 1.39 units/mg protein, indicating successful overexpression of the fabGJ genes in Pseudomonas putida KCTC1639. | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ | beta-oxidation pathway | Pseudomonas putida | 3-oxoacyl-[acyl-carrier protein] + NADPH + H+ | - |
? | |
| additional information | fabG overexpression tends to depress the biosynthesis of medium-chain-length polyhydroxyalkanoate due to the reversible conversion of (R)-3-hydroxyalkanoate monomer units into 3-ketoacyl-CoA | Pseudomonas putida | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-ketoacyl-ACP reductase | - |
Pseudomonas putida |
| 3-ketoacyl-acyl carrier protein reductase | - |
Pseudomonas putida |
| FabG | - |
Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Pseudomonas putida | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pseudomonas putida | predicted | - |
5.9 |
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