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Literature summary for 1.1.1.100 extracted from
- Production and purification of refolded recombinant Plasmodium falciparum beta-ketoacyl-ACP reductase from inclusion bodies (2005), Protein Expr. Purif., 42, 131-136.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) in inclusion bodies | Plasmodium falciparum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) solubilized from inclusion bodies and refolded is further purified by nickel affinity chromatography | Plasmodium falciparum |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| solubilization of overexpressed recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) inclusion bodies by 8 M urea, and refolding by dialysis, cation exchange chromatography, and dilution in presence of 0.001 mM NADPH in 20 mM sodium acetate, pH 5.3, 10% glycerol, and 0.05% Tween 20, the refolded enzyme shows 90-95% of soluble enzyme activity | Plasmodium falciparum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0017 | 0.0018 | recombinant refolded enzyme | Plasmodium falciparum |
| 0.0019 | - |
recombinant soluble enzyme | Plasmodium falciparum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetoacetyl-CoA + NADPH | - |
Plasmodium falciparum | 3-hydroxybutyryl-CoA + NADP+ | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-ketoacyl-ACP reductase | - |
Plasmodium falciparum |
| FabG | - |
Plasmodium falciparum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Plasmodium falciparum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Plasmodium falciparum | |
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