Cloned (Comment) | Organism |
---|---|
gene rhlG, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) or, as selenomethionine-labeled variant in Escherichia coli strain B834 in minimal medium | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
purified native and selenomethionine-labeled recombinant enzyme, hanging drop vapour diffusion method, 18°C, 2.5 mg/ml protein in 50 mm HEPES, pH 7.0, 5 mM Tris, pH 8.0, 0.15 mM ammonium sulfate, 6% PEG 4000, 0.1 M NaCl, 0.5 mM EDTA, and 0.5 mM DTT, mixed with reservoir solution containing 1 M HEPES, pH 7.0, 0.3 M ammonium sulfate, and 12% PEG 4000, plus 1 mM NADPH, X-ray diffraction structure determination and analysis at 2.3 A resolution | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
2 * 29000, recombinant enzyme, SDS-PAGE | Pseudomonas aeruginosa |
29000 | - |
4 * 29000, SDS-PAGE, tetrameric at higher enzyme concentrations | Pseudomonas aeruginosa |
50400 | - |
recombinant enzyme, gel filtration | Pseudomonas aeruginosa |
58400 | - |
recombinant enzyme, analytical ultracentrifugation | Pseudomonas aeruginosa |
116000 | - |
recombinant enzyme, analytical ultracentrifugation, tetrameric at higher enzyme concentrations | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas aeruginosa | the enzyme is essential in the rhamnolipid biosynthetic pathway of Pseudomonas aeruginosa | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
gene rhlG | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is essential in the rhamnolipid biosynthetic pathway of Pseudomonas aeruginosa | Pseudomonas aeruginosa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 29000, recombinant enzyme, SDS-PAGE | Pseudomonas aeruginosa |
tetramer | 4 * 29000, SDS-PAGE, tetrameric at higher enzyme concentrations | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
beta-ketoacyl reductase | - |
Pseudomonas aeruginosa |
RhlG | - |
Pseudomonas aeruginosa |