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Literature summary for 1.1.1.100 extracted from

  • Miller, D.J.; Zhang, Y.M.; Rock, C.O.; White, S.W.
    Structure of RhlG, an essential beta-ketoacyl reductase in the rhamnolipid biosynthetic pathway of Pseudomonas aeruginosa (2006), J. Biol. Chem., 281, 18025-18032.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene rhlG, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) or, as selenomethionine-labeled variant in Escherichia coli strain B834 in minimal medium Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
purified native and selenomethionine-labeled recombinant enzyme, hanging drop vapour diffusion method, 18°C, 2.5 mg/ml protein in 50 mm HEPES, pH 7.0, 5 mM Tris, pH 8.0, 0.15 mM ammonium sulfate, 6% PEG 4000, 0.1 M NaCl, 0.5 mM EDTA, and 0.5 mM DTT, mixed with reservoir solution containing 1 M HEPES, pH 7.0, 0.3 M ammonium sulfate, and 12% PEG 4000, plus 1 mM NADPH, X-ray diffraction structure determination and analysis at 2.3 A resolution Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
2 * 29000, recombinant enzyme, SDS-PAGE Pseudomonas aeruginosa
29000
-
4 * 29000, SDS-PAGE, tetrameric at higher enzyme concentrations Pseudomonas aeruginosa
50400
-
recombinant enzyme, gel filtration Pseudomonas aeruginosa
58400
-
recombinant enzyme, analytical ultracentrifugation Pseudomonas aeruginosa
116000
-
recombinant enzyme, analytical ultracentrifugation, tetrameric at higher enzyme concentrations Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas aeruginosa the enzyme is essential in the rhamnolipid biosynthetic pathway of Pseudomonas aeruginosa ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
gene rhlG
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is essential in the rhamnolipid biosynthetic pathway of Pseudomonas aeruginosa Pseudomonas aeruginosa ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 29000, recombinant enzyme, SDS-PAGE Pseudomonas aeruginosa
tetramer 4 * 29000, SDS-PAGE, tetrameric at higher enzyme concentrations Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
beta-ketoacyl reductase
-
Pseudomonas aeruginosa
RhlG
-
Pseudomonas aeruginosa