Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.100 extracted from

  • Wang, H.; Cronan, J.E.
    Only one of the two annotated Lactococcus lactis fabG genes encodes a functional beta-ketoacyl-acyl carrier protein reductase (2004), Biochemistry, 43, 11782-11789.
    View publication on PubMed
Search for more literature for 1.1.1.100

Cloned(Commentary)

Cloned (Comment) Organism
complementation of Escherichia coli fabG mutant with Lactobacillus lactis fabG genes shows that fabG1 encodes a functional beta-ketoacyl ACP reductase and fabG2 encodes an enzyme that has activity with the model substrate beta-ketobutyryl-CoA, but lacks the ability to support fatty acid synthesis both in vivo and in vitro Lactococcus lactis

Inhibitors

Inhibitors Comment Organism Structure
Acyl carrier protein beta-ketobutyryl-CoA reductase activity of FabG1 is inhibited, activity of FabG2 is unaffected Lactococcus lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.29
-
 beta-ketobutyryl-CoA pH 7.0, 30°C, FabG1 Lactococcus lactis
4.06
-
 beta-ketobutyryl-CoA pH 7.0, 30°C, FabG2 Lactococcus lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 x * 24000, recombinant methionine-labelled FabG2, SDS-PAGE Lactococcus lactis
25000
-
 x * 25000, recombinant methionine-labelled FabG1, SDS-PAGE Lactococcus lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Lactococcus lactis FabG1 is responsible for the first reductive step of the fatty acid synthetic cycle. FabG2 is inactive in fatty acid synthesis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
 ssp. lactis IL 1403
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-ketobutyryl-CoA + NADPH complementation of Escherichia coli fabG mutant with Lactobacillus lactis fabG genes shows that fabG1 encodes a functional beta-ketoacyl ACP reductase and fabG2 encodes an enzyme that has activity with the model substrate beta-ketobutyryl-CoA, but lacks the ability to support fatty acid synthesis both in vivo and in vitro Lactococcus lactis beta-hydroxybutyryl-CoA + NADP+
-
 ?
additional information FabG1 is responsible for the first reductive step of the fatty acid synthetic cycle. FabG2 is inactive in fatty acid synthesis Lactococcus lactis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 24000, recombinant methionine-labelled FabG2, SDS-PAGE Lactococcus lactis
? x * 25000, recombinant methionine-labelled FabG1, SDS-PAGE Lactococcus lactis

Synonyms

Synonyms Comment Organism
FabG1
-
 Lactococcus lactis