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Literature summary for 1.1.1.100 extracted from

  • Sheldon, P.S.; Kekwick, R.G.O.; Smith, C.G.; Sidebottom, C.; Slabas, A.R.
    3-Oxoacyl-[ACP] reductase from oil seed rape (Brassica napus) (1992), Biochim. Biophys. Acta, 1130, 151-159.
    View publication on PubMed

General Stability

General Stability Organism
inactivation by dilution can be partly prevented by inclusion of NADPH Brassica napus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
acetoacetyl-[acyl-carrier protein]
-
Brassica napus
0.023
-
NADPH + acetoacetyl-[acyl-carrier-protein] Brassica napus
0.035
-
N-(2-sulfanylethyl)acetamide
-
Brassica napus
0.261
-
acetoacetyl-CoA
-
Brassica napus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
gel filtration Brassica napus

Organism

Organism UniProt Comment Textmining
Brassica napus
-
oilseed
-

Purification (Commentary)

Purification (Comment) Organism
-
Brassica napus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
136000
-
purified enzyme, protein determination by calculation from absorption at 280 nm Brassica napus

Storage Stability

Storage Stability Organism
27°C, 100 mM potassium phosphate buffer, 13% loss of activity after 24 h Brassica napus
4°C, 100 mM potassium phosphate, 52% loss of activity after 24 h Brassica napus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-oxoacyl-[acyl-carrier protein] + NADPH
-
Brassica napus (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
?
acetoacetyl-CoA + NADPH + H+
-
Brassica napus D-beta-hydroxybutyryl-CoA + NADP+
-
?
acetoacetyl-N-cysteamine + NADPH
-
Brassica napus D-3-hydroxybutyryl-N-cysteamine + NADP+
-
?
acetoacetyl-[acyl-carrier protein] + NADPH
-
Brassica napus D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 20000-30000, SDS-PAGE Brassica napus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Brassica napus