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Literature summary for 1.1.1.10 extracted from

  • Zhao, H.T.; Endo, S.; Ishikura, S.; Chung, R.; Hogg, P.J.; Hara, A.; El-Kabbani, O.
    Structure/function analysis of a critical disulfide bond in the active site of L-xylulose reductase (2009), Cell. Mol. Life Sci., 66, 1570-1579.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
vapour diffusion method, using 15% PEG 8000, 0.05 M potassium phosphate and 0.1 M MES buffer, pH 6.5 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
cysteine the addition of cysteine (more than 2 mM) inactivates human L-xylulose reductase and is accompanied by a 10fold decrease in catalytic efficiency, the activity of the cysteine-inactivated enzyme is not recovered by the addition of 10 mM dithiothreitol and 2-mercaptoethanol Homo sapiens
potassium phosphate when the purified enzyme containing 2 mM 2-mercaptoethanol is diluted with 10 mM potassium phosphate pH 7.0, its diacetyl reductase activity is gradually decreased Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0945
-
diacetyl wild type enzyme, in 10 mM potassium phosphate buffer Homo sapiens
0.22
-
diacetyl mutant enzyme C138A, in 10 mM potassium phosphate buffer Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q7Z4W1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diacetyl + NADPH + H+
-
Homo sapiens acetoin + NADP+
-
?

Subunits

Subunits Comment Organism
dimer x-ray crystallography Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Homo sapiens