Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
vapour diffusion method, using 15% PEG 8000, 0.05 M potassium phosphate and 0.1 M MES buffer, pH 6.5 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cysteine | the addition of cysteine (more than 2 mM) inactivates human L-xylulose reductase and is accompanied by a 10fold decrease in catalytic efficiency, the activity of the cysteine-inactivated enzyme is not recovered by the addition of 10 mM dithiothreitol and 2-mercaptoethanol | Homo sapiens | |
potassium phosphate | when the purified enzyme containing 2 mM 2-mercaptoethanol is diluted with 10 mM potassium phosphate pH 7.0, its diacetyl reductase activity is gradually decreased | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0945 | - |
diacetyl | wild type enzyme, in 10 mM potassium phosphate buffer | Homo sapiens | |
0.22 | - |
diacetyl | mutant enzyme C138A, in 10 mM potassium phosphate buffer | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q7Z4W1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diacetyl + NADPH + H+ | - |
Homo sapiens | acetoin + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | x-ray crystallography | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |