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Literature summary for 1.1.1.10 extracted from
- Biochemical characterization of an L-Xylulose reductase from Neurospora crassa (2007), Appl. Environ. Microbiol., 73, 2001-2004.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His6 tag fusion protein | Neurospora crassa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0026 | - |
NADPH | - |
Neurospora crassa |  |
| 0.153 | - |
NADP+ | - |
Neurospora crassa | |
| 150 | - |
D-ribulose | - |
Neurospora crassa | |
| 1239 | - |
xylitol | - |
Neurospora crassa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-ribulose + NADPH | - |
Neurospora crassa | D-ribitol + NADP+ | - |
? | |
| L-xylulose + NADPH + H+ | - |
Neurospora crassa | L-xylitol + NADP+ | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
reductase activity | Neurospora crassa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 55 | 15°C: about 65% of maximal activity, 55°C: about 60% of maximal activity, reductase activity | Neurospora crassa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.6 | - |
xylitol | - |
Neurospora crassa | |
| 119 | - |
D-ribulose | - |
Neurospora crassa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
reductase activity | Neurospora crassa |
| 9 | - |
oxidase activity | Neurospora crassa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 8.5 | pH 4.5: about 55% of maximal activity, pH 8.5: about 70% of maximal activity, reductase activity | Neurospora crassa |
| 7 | 10.5 | pH 7.0: about 40% of maximal activity, pH 10.5: about 60% of maximal activity, oxidase activity | Neurospora crassa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme does not accept NAD+ | Neurospora crassa | |
| NADP+ | - |
Neurospora crassa | |
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