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Literature summary for 1.1.1.1 extracted from
- Characterization of alcohol dehydrogenase from Kangiella koreensis and its application to production of all-trans-retinol (2015), Biotechnol. Lett., 37, 849-856.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | under optimized conditions, the enzyme produces 600 mg all-trans-retinol per l after 3 h, with a conversion yield of 27.3% (w/w) and a productivity of 200 mg per l and h | Kangiella koreensis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Kangiella koreensis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39900 | - |
- |
Kangiella koreensis |
| 40000 | - |
- |
Kangiella koreensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Kangiella koreensis | C7R702 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 79.6 | - |
substrate butan-1-ol, pH 6.5, 60°C | Kangiella koreensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetaldehyde + NADH + H+ | 83.2% of the activity with butan-1-ol | Kangiella koreensis | ethanol + NAD+ | - |
? |  |
| all-trans retinal + NADH + H+ | 26.8% of the activity with butan-1-ol | Kangiella koreensis | all-trans retinol + NAD+ | - |
? | |
| butan-1-ol + NAD+ | - |
Kangiella koreensis | butanal + NADH + H+ | - |
? | |
| hexan-1-ol + NAD+ | 77.1% of the activity with butan-1-ol | Kangiella koreensis | hexanal + NADH + H+ | - |
? | |
| additional information | enzyme is an alcohol dehydrogenase with additional activity for all-trans-retinol, reaction of EC 1.1.1.184 | Kangiella koreensis | ? | - |
? | |
| propan-1-ol + NAD+ | 64.3% of the activity with butan-1-ol | Kangiella koreensis | propanal + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE, x * 39900, calculated | Kangiella koreensis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Kangiella koreensis | |
| NADH | - |
Kangiella koreensis | |
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Release 2023.1 (January 2023)
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