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Literature summary for 1.1.1.1 extracted from

  • Extance, J.; Crennell, S.J.; Eley, K.; Cripps, R.; Hough, D.W.; Danson, M.J.
    Structure of a bifunctional alcohol dehydrogenase involved in bioethanol generation in Geobacillus thermoglucosidasius (2013), Acta Crystallogr. Sect. D, 69, 2104-2115.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Parageobacillus thermoglucosidasius

Crystallization (Commentary)

Crystallization (Comment) Organism
structure of the alcohol dehydrogenase domain of the ADHE protein 2.5 A resolution and docking of the aldehyde dehydrogenase domain. The aldehyde dehydrogenase and alcohol dehydrogenase domains of a single ADHE may form dimers with different ADHE monomers rather than both with the same molecule Parageobacillus thermoglucosidasius

Protein Variants

Protein Variants Comment Organism
additional information expression of alcohol dehydrogenase domain alone, a minimal functional unit corresponds to amino acids 459-869 Parageobacillus thermoglucosidasius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.038
-
NADH wild-type, 60°C, pH 6.0, presence of 0.1 mM Zn2+ Parageobacillus thermoglucosidasius
0.062
-
NADH alcohol dehydrogenase domain, 60°C, pH 6.0, presence of 0.1 mM Zn2+ Parageobacillus thermoglucosidasius
34
-
acetaldehyde wild-type, 60°C, pH 6.0, presence of 0.1 mM Zn2+ Parageobacillus thermoglucosidasius
121
-
acetaldehyde alcohol dehydrogenase domain, 60°C, pH 6.0, presence of 0.1 mM Zn2+ Parageobacillus thermoglucosidasius

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ stimulation Parageobacillus thermoglucosidasius
Cu2+ stimulation Parageobacillus thermoglucosidasius
Fe2+ stimulation Parageobacillus thermoglucosidasius
Mg2+ stimulation Parageobacillus thermoglucosidasius
Mn2+ stimulation Parageobacillus thermoglucosidasius
additional information alcohol dehydrogenase activity is stimulated by a range of divalent metal ions, while aldehyde dehydrogenase activity is not Parageobacillus thermoglucosidasius
Zn2+ stimulation. Crystal structure of alcohol dehydrogenase domain contains 0.43 Zn atoms per protein monomer Parageobacillus thermoglucosidasius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48600
-
2 * 48600, alcohol dehydrogenase domain, SDS-PAGE. Unlike the native ADHE, the alcohol dehydrogenase domain alone does not assemble into spirosome structures Parageobacillus thermoglucosidasius
86000
-
alcohol dehydrogenase domain, gel filtration Parageobacillus thermoglucosidasius
96000
-
x * 96000, wild-type, SDS-PAGE Parageobacillus thermoglucosidasius
99000
-
alcohol dehydrogenase domain, dynamic light-scattering Parageobacillus thermoglucosidasius

Organism

Organism UniProt Comment Textmining
Parageobacillus thermoglucosidasius
-
-
-
Parageobacillus thermoglucosidasius C56-YS93
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Parageobacillus thermoglucosidasius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NADH + H+
-
Parageobacillus thermoglucosidasius ethanol + NAD+ + H+
-
?
acetaldehyde + NADH + H+
-
Parageobacillus thermoglucosidasius C56-YS93 ethanol + NAD+ + H+
-
?

Subunits

Subunits Comment Organism
? x * 96000, wild-type, SDS-PAGE Parageobacillus thermoglucosidasius
dimer 2 * 48600, alcohol dehydrogenase domain, SDS-PAGE. Unlike the native ADHE, the alcohol dehydrogenase domain alone does not assemble into spirosome structures Parageobacillus thermoglucosidasius

Synonyms

Synonyms Comment Organism
bifunctional alcohol/aldehyde dehydrogenase catalyzes reactions of EC 1.1.1.1 and EC 1.2.1.10 Parageobacillus thermoglucosidasius

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
83
-
acetaldehyde wild-type, 60°C, pH 6.0, presence of 0.1 mM Zn2+ Parageobacillus thermoglucosidasius
350
-
acetaldehyde alcohol dehydrogenase domain, 60°C, pH 6.0, presence of 0.1 mM Zn2+ Parageobacillus thermoglucosidasius

Cofactor

Cofactor Comment Organism Structure
NADH
-
Parageobacillus thermoglucosidasius