Cloned (Comment) | Organism |
---|---|
- |
Parageobacillus thermoglucosidasius |
Crystallization (Comment) | Organism |
---|---|
structure of the alcohol dehydrogenase domain of the ADHE protein 2.5 A resolution and docking of the aldehyde dehydrogenase domain. The aldehyde dehydrogenase and alcohol dehydrogenase domains of a single ADHE may form dimers with different ADHE monomers rather than both with the same molecule | Parageobacillus thermoglucosidasius |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression of alcohol dehydrogenase domain alone, a minimal functional unit corresponds to amino acids 459-869 | Parageobacillus thermoglucosidasius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
NADH | wild-type, 60°C, pH 6.0, presence of 0.1 mM Zn2+ | Parageobacillus thermoglucosidasius | |
0.062 | - |
NADH | alcohol dehydrogenase domain, 60°C, pH 6.0, presence of 0.1 mM Zn2+ | Parageobacillus thermoglucosidasius | |
34 | - |
acetaldehyde | wild-type, 60°C, pH 6.0, presence of 0.1 mM Zn2+ | Parageobacillus thermoglucosidasius | |
121 | - |
acetaldehyde | alcohol dehydrogenase domain, 60°C, pH 6.0, presence of 0.1 mM Zn2+ | Parageobacillus thermoglucosidasius |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | stimulation | Parageobacillus thermoglucosidasius | |
Cu2+ | stimulation | Parageobacillus thermoglucosidasius | |
Fe2+ | stimulation | Parageobacillus thermoglucosidasius | |
Mg2+ | stimulation | Parageobacillus thermoglucosidasius | |
Mn2+ | stimulation | Parageobacillus thermoglucosidasius | |
additional information | alcohol dehydrogenase activity is stimulated by a range of divalent metal ions, while aldehyde dehydrogenase activity is not | Parageobacillus thermoglucosidasius | |
Zn2+ | stimulation. Crystal structure of alcohol dehydrogenase domain contains 0.43 Zn atoms per protein monomer | Parageobacillus thermoglucosidasius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48600 | - |
2 * 48600, alcohol dehydrogenase domain, SDS-PAGE. Unlike the native ADHE, the alcohol dehydrogenase domain alone does not assemble into spirosome structures | Parageobacillus thermoglucosidasius |
86000 | - |
alcohol dehydrogenase domain, gel filtration | Parageobacillus thermoglucosidasius |
96000 | - |
x * 96000, wild-type, SDS-PAGE | Parageobacillus thermoglucosidasius |
99000 | - |
alcohol dehydrogenase domain, dynamic light-scattering | Parageobacillus thermoglucosidasius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Parageobacillus thermoglucosidasius | - |
- |
- |
Parageobacillus thermoglucosidasius C56-YS93 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Parageobacillus thermoglucosidasius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NADH + H+ | - |
Parageobacillus thermoglucosidasius | ethanol + NAD+ + H+ | - |
? | |
acetaldehyde + NADH + H+ | - |
Parageobacillus thermoglucosidasius C56-YS93 | ethanol + NAD+ + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 96000, wild-type, SDS-PAGE | Parageobacillus thermoglucosidasius |
dimer | 2 * 48600, alcohol dehydrogenase domain, SDS-PAGE. Unlike the native ADHE, the alcohol dehydrogenase domain alone does not assemble into spirosome structures | Parageobacillus thermoglucosidasius |
Synonyms | Comment | Organism |
---|---|---|
bifunctional alcohol/aldehyde dehydrogenase | catalyzes reactions of EC 1.1.1.1 and EC 1.2.1.10 | Parageobacillus thermoglucosidasius |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
83 | - |
acetaldehyde | wild-type, 60°C, pH 6.0, presence of 0.1 mM Zn2+ | Parageobacillus thermoglucosidasius | |
350 | - |
acetaldehyde | alcohol dehydrogenase domain, 60°C, pH 6.0, presence of 0.1 mM Zn2+ | Parageobacillus thermoglucosidasius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Parageobacillus thermoglucosidasius |