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Literature summary for 1.1.1.1 extracted from

  • Hirakawa, H.; Kamiya, N.; Kawarabayashi, Y.; Nagamune, T.
    Properties of an alcohol dehydrogenase from the hyperthermophilic archaeon Aeropyrum pernix K1 (2004), J. Biosci. Bioeng., 97, 202-206.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Aeropyrum pernix

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
NADH pH 8.0, 60°C Aeropyrum pernix
0.001
-
NAD+ pH 8.0, 60°C Aeropyrum pernix
0.131
-
4-methoxyphenylacetone pH 8.0, 60°C Aeropyrum pernix
0.147
-
1-Hexanol pH 8.0, 60°C Aeropyrum pernix
0.147
-
2-decanone pH 8.0, 60°C Aeropyrum pernix
0.215
-
2-nonanone pH 8.0, 60°C Aeropyrum pernix
0.286
-
2-Octanone pH 8.0, 60°C Aeropyrum pernix
0.333
-
benzaldehyde pH 8.0, 60°C Aeropyrum pernix
0.396
-
1-Pentanol pH 8.0, 60°C Aeropyrum pernix
0.596
-
1-butanol pH 8.0, 60°C Aeropyrum pernix
0.694
-
tert-butyl acetoacetate pH 8.0, 60°C Aeropyrum pernix
0.703
-
Cyclohexanol pH 8.0, 60°C Aeropyrum pernix
0.752
-
2-Pentanol pH 8.0, 60°C Aeropyrum pernix
1.03
-
1-propanol pH 8.0, 60°C Aeropyrum pernix
1.05
-
2-butanol pH 8.0, 60°C Aeropyrum pernix
1.13
-
4-methoxybenzyl alcohol pH 8.0, 60°C Aeropyrum pernix
1.16
-
2-Heptanone pH 8.0, 60°C Aeropyrum pernix
1.39
-
cyclohexanone pH 8.0, 60°C Aeropyrum pernix
2.44
-
2-propanol pH 8.0, 60°C Aeropyrum pernix
5.01
-
2-Hexanone pH 8.0, 60°C Aeropyrum pernix
5.15
-
2-Pentanone pH 8.0, 60°C Aeropyrum pernix
5.43
-
benzyl alcohol pH 8.0, 60°C Aeropyrum pernix
13.7
-
ethanol pH 8.0, 60°C Aeropyrum pernix

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39570
-
4 * 39570, calculated from sequence Aeropyrum pernix
40000
-
4 * 40000, SDS-PAGE Aeropyrum pernix
160000
-
gel filtration Aeropyrum pernix

Organism

Organism UniProt Comment Textmining
Aeropyrum pernix Q9Y9P9
-
-
Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1 Q9Y9P9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aeropyrum pernix

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-butanol + NAD+
-
Aeropyrum pernix butanal + NADH + H+
-
?
1-butanol + NAD+
-
Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1 butanal + NADH + H+
-
?
1-hexanol + NAD+
-
Aeropyrum pernix 1-hexanal + NADH + H+
-
?
1-pentanol + NAD+
-
Aeropyrum pernix 1-pentanal + NADH + H+
-
?
1-propanol + NAD+
-
Aeropyrum pernix propanal + NADH + H+
-
?
1-propanol + NAD+
-
Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1 propanal + NADH + H+
-
?
2-butanol + NAD+
-
Aeropyrum pernix 2-butanone + NADH + H+
-
?
2-butanol + NAD+
-
Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1 2-butanone + NADH + H+
-
?
2-decanone + NADH
-
Aeropyrum pernix (S)-2-decanol + NAD+ + H+ 92% enantiomeric excess ?
2-heptanone + NADH
-
Aeropyrum pernix (S)-2-heptanol + NAD+ + H+ 79% enantiomeric excess ?
2-hexanone + NADH
-
Aeropyrum pernix (S)-2-hexanol + NAD+ + H+ 37% enantiomeric excess ?
2-nonanone + NADH
-
Aeropyrum pernix (S)-2-nonanol + NAD+ + H+ 95% enantiomeric excess ?
2-octanone + NADH
-
Aeropyrum pernix (S)-2-octanol + NAD+ + H+ 92% enantiomeric excess ?
2-pentanol + NAD+
-
Aeropyrum pernix 2-pentanone + NADH + H+
-
?
2-pentanone + NADH + H+
-
Aeropyrum pernix (S)-2-pentanol + NAD+ 60% enantiomeric excess ?
2-propanol + NAD+
-
Aeropyrum pernix acetone + NADH + H+
-
?
2-propanol + NAD+
-
Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1 acetone + NADH + H+
-
?
4-methoxybenzyl alcohol + NAD+
-
Aeropyrum pernix 4-methoxybenzaldehyde + NADH + H+
-
?
4-methoxyphenylacetone + NADH
-
Aeropyrum pernix (2S)-1-(4-methoxyphenyl)propan-2-ol + NAD+ + H+
-
?
benzyl alcohol + NAD+ i.e. phenylmethanol Aeropyrum pernix benzaldehyde + NADH + H+
-
?
cyclohexanol + NAD+
-
Aeropyrum pernix cyclohexanone + NADH + H+
-
?
ethanol + NAD+
-
Aeropyrum pernix acetaldehyde + NADH + H+
-
?
additional information the enzyme shows broad substrate specificity and prefers aliphatic alcohols and ketones. There are no large differences in the reactivities between primary and secondary alcohols. The enzyme produces (S)-alcohols from the corresponding ketones. The values of the enantiomeric excess increase with the increase of chain length except for the reduction of 2-hexanone. The highest enantioselectivity is shown with the reduction of 2-nonanone Aeropyrum pernix ?
-
?
additional information the enzyme shows broad substrate specificity and prefers aliphatic alcohols and ketones. There are no large differences in the reactivities between primary and secondary alcohols. The enzyme produces (S)-alcohols from the corresponding ketones. The values of the enantiomeric excess increase with the increase of chain length except for the reduction of 2-hexanone. The highest enantioselectivity is shown with the reduction of 2-nonanone Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1 ?
-
?
tert-butyl acetoacetate + NADH + H+
-
Aeropyrum pernix ?
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 40000, SDS-PAGE Aeropyrum pernix
homotetramer 4 * 39570, calculated from sequence Aeropyrum pernix

Synonyms

Synonyms Comment Organism
APE2239
-
Aeropyrum pernix

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
catalytic activity increased up to 95°C Aeropyrum pernix

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
30 min, no loss of activity Aeropyrum pernix
80
-
30 min, about 20% loss of activity Aeropyrum pernix
98
-
the enzyme maintains 24% of the original catalytic activity after incubation for 30 min Aeropyrum pernix

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.071
-
4-methoxyphenylacetone pH 8.0, 60°C Aeropyrum pernix
0.072
-
tert-butyl acetoacetate pH 8.0, 60°C Aeropyrum pernix
0.23
-
ethanol pH 8.0, 60°C Aeropyrum pernix
0.24
-
2-propanol pH 8.0, 60°C Aeropyrum pernix
0.26
-
1-propanol pH 8.0, 60°C Aeropyrum pernix
0.37
-
1-Hexanol pH 8.0, 60°C Aeropyrum pernix
0.4
-
NAD+ pH 8.0, 60°C Aeropyrum pernix
0.4
-
2-decanone pH 8.0, 60°C Aeropyrum pernix
0.41
-
NADH pH 8.0, 60°C Aeropyrum pernix
0.41
-
1-butanol pH 8.0, 60°C Aeropyrum pernix
0.45
-
1-Pentanol pH 8.0, 60°C Aeropyrum pernix
0.48
-
2-butanol pH 8.0, 60°C Aeropyrum pernix
0.52
-
Cyclohexanol pH 8.0, 60°C Aeropyrum pernix
0.6
-
4-methoxybenzyl alcohol pH 8.0, 60°C Aeropyrum pernix
0.6
-
2-Pentanol pH 8.0, 60°C Aeropyrum pernix
0.7 1 2-nonanone pH 8.0, 60°C Aeropyrum pernix
0.73
-
2-Heptanone pH 8.0, 60°C Aeropyrum pernix
0.74
-
2-Octanone pH 8.0, 60°C Aeropyrum pernix
0.77
-
2-Pentanone pH 8.0, 60°C Aeropyrum pernix
1.02
-
benzyl alcohol pH 8.0, 60°C Aeropyrum pernix
1.08
-
2-Hexanone pH 8.0, 60°C Aeropyrum pernix
1.22
-
benzaldehyde pH 8.0, 60°C Aeropyrum pernix
1.27
-
cyclohexanone pH 8.0, 60°C Aeropyrum pernix

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
reduction of 2-pentanone Aeropyrum pernix
10.5
-
oxidation of 2-pentanol Aeropyrum pernix

pH Range

pH Minimum pH Maximum Comment Organism
7 9.6 pH 7.0: about 70% of maximal activity, pH 9.6: about 50% of maximal activity, reduction of 2-pentanone Aeropyrum pernix
9.6 11.5 pH 9.5: about 40% of maximal activity, pH 11.5: about 30% of maximal activity, oxidation of 2-pentanol Aeropyrum pernix

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.017
-
ethanol pH 8.0, 60°C Aeropyrum pernix
0.097
-
2-propanol pH 8.0, 60°C Aeropyrum pernix
0.1
-
tert-butyl acetoacetate pH 8.0, 60°C Aeropyrum pernix
0.15
-
2-Pentanone pH 8.0, 60°C Aeropyrum pernix
0.189
-
benzyl alcohol pH 8.0, 60°C Aeropyrum pernix
0.22
-
2-Hexanone pH 8.0, 60°C Aeropyrum pernix
0.25
-
1-propanol pH 8.0, 60°C Aeropyrum pernix
0.46
-
2-butanol pH 8.0, 60°C Aeropyrum pernix
0.53
-
4-methoxybenzyl alcohol pH 8.0, 60°C Aeropyrum pernix
0.54
-
4-methoxyphenylacetone pH 8.0, 60°C Aeropyrum pernix
0.62
-
2-Heptanone pH 8.0, 60°C Aeropyrum pernix
0.69
-
1-butanol pH 8.0, 60°C Aeropyrum pernix
0.73
-
Cyclohexanol pH 8.0, 60°C Aeropyrum pernix
0.79
-
2-Pentanol pH 8.0, 60°C Aeropyrum pernix
0.91
-
cyclohexanone pH 8.0, 60°C Aeropyrum pernix
1.1
-
1-Pentanol pH 8.0, 60°C Aeropyrum pernix
2.5
-
1-Hexanol pH 8.0, 60°C Aeropyrum pernix
2.6
-
2-Octanone pH 8.0, 60°C Aeropyrum pernix
2.7
-
2-decanone pH 8.0, 60°C Aeropyrum pernix
3.3
-
2-nonanone pH 8.0, 60°C Aeropyrum pernix
3.66
-
benzaldehyde pH 8.0, 60°C Aeropyrum pernix
380
-
NAD+ pH 8.0, 60°C Aeropyrum pernix
1000
-
NADH pH 8.0, 60°C Aeropyrum pernix