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Literature summary for 1.1.1.1 extracted from

  • Kube, J.; Brokamp, C.; Machielsen, R.; van der Oost, J.; Maerkl, H.
    Influence of temperature on the production of an archaeal thermoactive alcohol dehydrogenase from Pyrococcus furiosus with recombinant Escherichia coli (2006), Extremophiles, 10, 221-227.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information without heat treatment the AdhC enzyme shows no detectable activity, but after 10 min at higher temperatures the activity is revealed. The highest activity is measured after 10 min at 100°C Pyrococcus furiosus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli. The correct folding of the AdhC enzyme from hyperthermophilic Pyrococcus furiosus in mesophilic recombinant Escherichia coli is greatly influenced by the cultivation temperature. When grown at temperatures above the optimal growth temperature, Escherichia coli produces heat shock proteins to prevent protein aggregation. Heat shock proteins are known for their chaperonin activity, i.e., they help the protein folding and are responsible for an efficient protein quality control. When heated at 45°C for 2.5 h prior to induction, an increase of the activity is monitored compared to the standard cultivation at 37°C. The fast increase to 42°C yields more active enzyme than the slow increase to 42°C. This suggests that heat shock proteins either assist in the correct folding of the AdhC, or maybe even allow for resolubilization of (partially) denatured molecules. The cultivation at 45°C is not successful Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
4 * 38000, calculated from sequence Pyrococcus furiosus
150000
-
gel filtration Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8U259
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-butanediol + NAD+
-
Pyrococcus furiosus acetoin + NADH + H+
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 38000, calculated from sequence Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
AdhC
-
Pyrococcus furiosus
PF0991 protein
-
Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
assay at Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
assay at Pyrococcus furiosus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pyrococcus furiosus