Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21DE3pLysS cells | Euglena gracilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
NAD+ | at pH 9.0 in bis-Tris propane buffer | Euglena gracilis | |
0.39 | - |
NAD+ | at pH 7.0 and 25°C | Euglena gracilis | |
3.1 | - |
ethanol | at pH 9.0 in bis-Tris propane buffer | Euglena gracilis | |
3.2 | - |
ethanol | at pH 7.0 and 25°C | Euglena gracilis | |
20.3 | - |
Isopropanol | at pH 7.0 and 25°C | Euglena gracilis | |
197 | - |
Isopentanol | at pH 7.0 and 25°C | Euglena gracilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | essential for activity, in the absence of Mg2+ in the kinetic assay no ADH activity is detected | Euglena gracilis | |
Zn2+ | 20% of activity is obtained when replacing Mg2+ with 5 mM ZnSO4 | Euglena gracilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
SDS-PAGE | Euglena gracilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euglena gracilis | B8QU18 | - |
- |
Euglena gracilis Z | B8QU18 | - |
- |
Purification (Comment) | Organism |
---|---|
Talon Co2+-affinity column chromatography | Euglena gracilis |
Source Tissue | Comment | Organism | Textmining |
---|
Storage Stability | Organism |
---|---|
-20°C, in the presence of 50% (v/v) glycerol and at highly concentrated solutions (0.75-1 mg protein/ml), 3 days, 40-50% loss of activity | Euglena gracilis |
4°C, in 3.2 M ammonium sulfate or 50% glycerol, 1 day, rapid loss of activity | Euglena gracilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanol + NAD+ | - |
Euglena gracilis | acetaldehyde + NADH + H+ | - |
? | |
ethanol + NAD+ | - |
Euglena gracilis Z | acetaldehyde + NADH + H+ | - |
? | |
isopentanol + NAD+ | low affinity | Euglena gracilis | ? + NADH + H+ | - |
? | |
isopentanol + NAD+ | low affinity | Euglena gracilis Z | ? + NADH + H+ | - |
? | |
isopropanol + NAD+ | low affinity | Euglena gracilis | acetone + NADH + H+ | - |
? | |
isopropanol + NAD+ | low affinity | Euglena gracilis Z | acetone + NADH + H+ | - |
? | |
additional information | no activity is detected using 1 mM NADP+ | Euglena gracilis | ? | - |
? | |
additional information | no activity is detected using 1 mM NADP+ | Euglena gracilis Z | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADH | - |
Euglena gracilis |
medium-chain NAD+-dependent ADH | - |
Euglena gracilis |
NAD+-ADH | - |
Euglena gracilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | for ethanol oxidation | Euglena gracilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | with 100 mM diphosphate buffer at pH 9.0, the enzyme only exhibits 49% of activity, a 50% decrease is obtained using 50 mM bis-Tris propane buffer at pH 7.0-7.5 and 9.0, whereas at pH 8 and 8.5 only a 10% reduction is found | Euglena gracilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | specific for NAD+ | Euglena gracilis |