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Literature summary for 1.1.1.1 extracted from

  • Kizaki, N.; Yasohara, Y.; Nagashima, N.; Hasegawa, J.
    Characterization of novel alcohol dehydrogenase of Devosia riboflavina involved in stereoselective reduction of 3-pyrrolidinone derivatives (2008), J. Mol. Catal. B, 51, 73-80.
No PubMed abstract available
Search for more literature for 1.1.1.1

Activating Compound

Activating Compound Comment Organism Structure
iodoacetate 1 mM, 1.1fold activation Devosia riboflavina

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1 mM, 6% inhibition Devosia riboflavina
2,2'-bipyridyl 1 mM, 11% inhibition Devosia riboflavina
2,4-dinitrophenol 1 mM, complete inhibition Devosia riboflavina
2-mercaptoethanol 1 mM, 19% inhibition Devosia riboflavina
AgNO3 1 mM, 37% inhibition Devosia riboflavina
BaCl2 1 mM, complete inhibition Devosia riboflavina
dithiothreitol 1 mM, 6% inhibition Devosia riboflavina
EDTA 1 mM, 31% inhibition Devosia riboflavina
FeSO4 1 mM, complete inhibition Devosia riboflavina
HgCl2 1 mM, complete inhibition Devosia riboflavina
N-ethylmaleimide 1 mM, 11% inhibition Devosia riboflavina
PbCl2 1 mM, complete inhibition Devosia riboflavina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.58
-
 N-benzyl-3-pyrrolidinone
-
 Devosia riboflavina

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 1 mM CuSO4, 1.7fold activation Devosia riboflavina
Li+ 1 mM LiCl, 1.1fold activation Devosia riboflavina
Ni2+ 1 mM NiSO4, 1.2fold activation Devosia riboflavina
Zn2+ 1 mM ZnSO4, 1.3fold activation Devosia riboflavina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 2 * 28000, SDS-PAGE Devosia riboflavina
58000
-
 gel filtration Devosia riboflavina

Organism

Organism UniProt Comment Textmining
Devosia riboflavina
-
 KNK10702
-
Devosia riboflavina KNK10702
-
 KNK10702
-

Purification (Commentary)

Purification (Comment) Organism
-
 Devosia riboflavina

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.12
-
-
 Devosia riboflavina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-2-butanol + NAD+ 55% of activity with N-benzyl-3-pyrrolidinol Devosia riboflavina 2-butanone + NADH + H+
-
 ?
(R)-2-butanol + NAD+ 55% of activity with N-benzyl-3-pyrrolidinol Devosia riboflavina KNK10702 2-butanone + NADH + H+
-
 ?
1-octanol + NAD+ 6% of activity with N-benzyl-3-pyrrolidinol Devosia riboflavina 1-octanal + NADH + H+
-
 ?
1-phenyl-2-butanone + NADH + H+ 1.5fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina 1-phenylbutan-2-ol + NAD+
-
 ?
1-phenyl-2-butanone + NADH + H+ 1.5fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina KNK10702 1-phenylbutan-2-ol + NAD+
-
 ?
2-acetylpyrrole + NADH + H+ 70% of activity with N-benzyl-3-pyrrolidinone Devosia riboflavina 1-(1H-pyrrol-2-yl)ethanol + NAD+
-
 ?
2-butanone + NADH + H+ 2.8fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina butan-2-ol + NAD+
-
 ?
2-hexanone + NADH + H+ 50.4fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina hexan-2-ol + NAD+
-
 ?
2-nitrobenzaldehyde + NADH + H+ 33% of activity with N-benzyl-3-pyrrolidinone Devosia riboflavina (2-nitrophenyl)methanol + NAD+
-
 ?
2-oxo-butyric acid + NADH + H+ 15% of activity with N-benzyl-3-pyrrolidinone Devosia riboflavina 2-hydroxybutyric acid + NAD+
-
 ?
2-pentanol + NAD+ 45% of activity with N-benzyl-3-pyrrolidinol Devosia riboflavina 2-pentanone + NADH + H+
-
 ?
2-pentanol + NAD+ 45% of activity with N-benzyl-3-pyrrolidinol Devosia riboflavina KNK10702 2-pentanone + NADH + H+
-
 ?
3-acetylpyridine + NADH + H+ 7.9fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina 1-pyridin-3-ylethanol + NAD+
-
 ?
3-phenylpropionaldehyde + NADH + H+ 6.9fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina 3-phenylpropanol + NAD+
-
 ?
4-acetylpyridine + NADH + H+ 64.5fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina 1-pyridin-4-ylethanol + NAD+
-
 ?
acetone + NADH + H+ 1.9fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina propan-2-ol + NAD+
-
 ?
acetophenone + NADH + H+ 1.8fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina 1-phenylethanol + NAD+
-
 ?
benzaldehyde + NADH + H+ 21% of activity with N-benzyl-3-pyrrolidinone Devosia riboflavina benzyl alcohol + NAD+
-
 ?
benzoin + NADH + H+ 13% of activity with N-benzyl-3-pyrrolidinone Devosia riboflavina 1,2-diphenylethane-1,2-diol
-
 ?
benzyloxycarbonyl-3-pyrrolidinone + NADH + H+ production with 99.6% enantiomeric excess Devosia riboflavina (S)-benzyloxycarbonyl-3-pyrrolidinol + NAD+
-
 ?
chloroacetone + NADH + H+ 41.7fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina 1-chloropropan-2-ol + NAD+
-
 ?
diacetyl + NADH + H+ 16.1fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina ?
-
 ?
diacetyl + NADH + H+ 16.1fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina KNK10702 ?
-
 ?
dihydro-4,4-dimethyl-2,3-furandione + NADH + H+ 13.1fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina ?
-
 ?
ethyl 4-chloroacetoacetate + NADH + H+ 6.8fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina ?
-
 ?
ethyl acetoacetate + NADH + H+ 42.4fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina ?
-
 ?
glutaraldehyde + NADH + H+ 70.8fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina ?
-
 ?
hexanal + NADH + H+ 22.3fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina n-hexanol + NAD+
-
 ?
levulinic acid + NADH + H+ 11% of activity with N-benzyl-3-pyrrolidinone Devosia riboflavina ?
-
 ?
m-chlorophenacyl chloride + NADH + H+ 4.6fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina 2-chloro-1-(3-chlorophenyl)ethanol + NAD+
-
 ?
methyl glyoxal + NADH + H+ 3.5fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina ?
-
 ?
methyl pyruvate + NADH + H+ 18.9fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina methyl 2-hydroxypropanoate + NAD+
-
 ?
additional information when NADH is replaced with NADPH, the reaction rate is reduced by 0.6% Devosia riboflavina ?
-
 ?
additional information when NADH is replaced with NADPH, the reaction rate is reduced by 0.6% Devosia riboflavina KNK10702 ?
-
 ?
N-benzyl-3-piperidone + NADH + H+ 2.3fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina N-benzylpiperidin-3-ol + NAD+
-
 ?
N-benzyl-3-pyrrolidinone + NADH + H+ (R)-stereoselectivity of the reduction carried out with the heat-treated cells Devosia riboflavina (R)-N-benzyl-3-pyrrolidinol + NAD+
-
 r
N-benzyl-3-pyrrolidinone + NADH + H+
-
 Devosia riboflavina (S)-N-benzyl-3-pyrrolidinol + NAD+
-
 r
N-benzyl-4-piperidone + NADH + H+ 10.7fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina N-benzylpiperidin-4-ol + NAD+
-
 ?
n-butyraldehyde + NADH + H+ 7.3fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina n-butanol + NAD+
-
 ?
N-tert-butoxycarbonyl-3-pyrrolidinone + NADH + H+ production of (S)-N-tert-butoxycarbonyl-3-pyrrolidinol with 99.6% enantiomeric excess Devosia riboflavina (S)-N-tert-butoxycarbonyl-3-pyrrolidinol + NAD+
-
 ?
oxalacetic acid + NADH + H+ 14% of activity with N-benzyl-3-pyrrolidinone Devosia riboflavina ?
-
 ?
p-chlorobenzaldehyde + NADH + H+ 1.2fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina p-chlorobenzyl alcohol + NAD+
-
 ?
p-nitrobenzyloxycarbonyl-3-pyrrolidinone + NADH + H+ production with 90.7% enantiomeric excess Devosia riboflavina (S)-N-p-nitrobenzyloxycarbonyl-3-pyrrolidinol + NAD+
-
 ?
phenylethanol + NAD+ 15% of activity with N-benzyl-3-pyrrolidinol Devosia riboflavina phenylacetaldehyde + NADH + H+
-
 ?
propiophenone + NADH + H+ 27% of activity with N-benzyl-3-pyrrolidinone Devosia riboflavina 1-phenylpropan-1-ol + NAD+
-
 ?
pyridine-3-aldehyde + NADH + H+ 82% of activity with N-benzyl-3-pyrrolidinone Devosia riboflavina pyridin-3-yl ethanol + NAD+
-
 ?
pyridine-4-aldehyde + NADH + H+ 4.4fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina pyridin-4-yl methanol + NAD+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 28000, SDS-PAGE Devosia riboflavina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Devosia riboflavina

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 65 30°C: 36% of maximal activity, 65°C: 24% of maximal activity Devosia riboflavina

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 1 h, 90 mM potassium phosphate buffer, pH 6.5, stable Devosia riboflavina
45
-
 1 h, 90 mM potassium phosphate buffer, pH 6.5, 70% loss of activity Devosia riboflavina
50
-
 1 h, 90 mM potassium phosphate buffer, pH 6.5, complete loss of activity Devosia riboflavina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.7
-
-
 Devosia riboflavina