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Literature summary for 1.1.1.1 extracted from

  • Li, G.Y.; Huang, K.L.; Jiang, Y.R.; Yang, D.L.; Ding, P.
    Preparation and characterization of Saccharomyces cerevisiae alcohol dehydrogenase immobilized on magnetic nanoparticles (2008), Int. J. Biol. Macromol., 42, 405-412.
    View publication on PubMed

General Stability

General Stability Organism
enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde shows enhanced thermal stability and good durability in the repeated use after recovered by magnetic separations. Within 7 cycles of usage, the remaining activity is about 100%, 89.15%, 79.42%, 69.50%, 62.80%, 56.48%, and 48.26% of the first use Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
24.45
-
phenylglyoxylic acid soluble enzyme Saccharomyces cerevisiae
37.55
-
phenylglyoxylic acid enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phenylglyoxylic acid + NADH + H+ enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde retains 48.77% activity of its original activity Saccharomyces cerevisiae hydroxy(phenyl)acetic acid + NAD+
-
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Synonyms

Synonyms Comment Organism
SCAD
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
soluble enzyme Saccharomyces cerevisiae
40
-
enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
60 min, about 35% loss of activity, soluble enzyme and enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde Saccharomyces cerevisiae
65
-
60 min, about 80% loss of activity of soluble enzyme, about 55% loss of activity of enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
soluble enzyme Saccharomyces cerevisiae
7.5
-
enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde Saccharomyces cerevisiae