Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.1 extracted from

  • Kotrbova-Kozak, A.; Kotrba, P.; Inui, M.; Sajdok, J.; Yukawa, H.
    Transcriptionally regulated adhA gene encodes alcohol dehydrogenase required for ethanol and n-propanol utilization in Corynebacterium glutamicum R (2007), Appl. Microbiol. Biotechnol., 76, 1347-1356.
    View publication on PubMed
Search for more literature for 1.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Corynebacterium glutamicum R

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.59
-
 propanal 33°C, pH 7 Corynebacterium glutamicum R
0.92
-
 acetaldehyde 33°C, pH 7 Corynebacterium glutamicum R
2.01
-
 propanone 33°C, pH 7 Corynebacterium glutamicum R
3.04
-
 methanol 33°C, pH 8 Corynebacterium glutamicum R
4.24
-
 Butanal 33°C, pH 7 Corynebacterium glutamicum R
6.8
-
 ethanol 33°C, pH 8 Corynebacterium glutamicum R
7.49
-
 n-Propanol 33°C, pH 8 Corynebacterium glutamicum R
10.3
-
 n-Pentanal 33°C, pH 8 Corynebacterium glutamicum R
11.9
-
 2-butanol 33°C, pH 8 Corynebacterium glutamicum R
19.6
-
 2-propanol 33°C, pH 8 Corynebacterium glutamicum R
35.1
-
 n-butanol 33°C, pH 8 Corynebacterium glutamicum R

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36900
-
 2 * 36900, SDS-PAGE Corynebacterium glutamicum R
81500
-
 gel filtration Corynebacterium glutamicum R

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum R
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Corynebacterium glutamicum R

Source Tissue

Source Tissue Comment Organism Textmining
culture condition adhA transcription is induced by ethanol or n-propanol, adhA transcription is subject to glucose catabolite repression. Accordingly, both induction of AdhA activity and ethanol utilization are detected only after depletion of glucose Corynebacterium glutamicum R
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-butanol + NAD+
-
 Corynebacterium glutamicum R 2-butanone + NADH + H+
-
 ?
2-propanol + NAD+
-
 Corynebacterium glutamicum R propanone + NADH + H+
-
 r
ethanol + NAD+ no activity with NADP+, in reverse reaction no activity with NADPH Corynebacterium glutamicum R acetaldehyde + NADH + H+
-
 r
methanol + NAD+
-
 Corynebacterium glutamicum R formaldehyde + NADH + H+
-
 ?
n-butanal + NADH + H+
-
 Corynebacterium glutamicum R n-butanol + NAD+
-
 ?
n-butanol + NAD+
-
 Corynebacterium glutamicum R n-butanal + NADH + H+
-
 ?
n-pentanol + NAD+
-
 Corynebacterium glutamicum R pentanal + NADH + H+
-
 ?
n-propanol + NAD+
-
 Corynebacterium glutamicum R propanal + NADH + H+
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 36900, SDS-PAGE Corynebacterium glutamicum R

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 reaction with ethanol + NAD+ Corynebacterium glutamicum R

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7 7.3 reaction with acetaldehyde + NADH Corynebacterium glutamicum R
7.7 8.6 reaction with ethanol + NAD+ Corynebacterium glutamicum R