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Literature summary for 1.1.1.1 extracted from

  • Chong, P.K.; Burja, A.M.; Radianingtyas, H.; Fazeli, A.; Wright, P.C.
    Proteome and transcriptional analysis of ethanol-grown Sulfolobus solfataricus P2 reveals ADH2, a potential alcohol dehydrogenase (2007), J. Proteome Res., 6, 3985-3994.
    View publication on PubMed
Search for more literature for 1.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star (DE3) cells Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
 n-Propanol 2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C Saccharolobus solfataricus
0.44
-
 benzylalcohol 2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C Saccharolobus solfataricus
1.33
-
 ethanol 2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C Saccharolobus solfataricus
2.4
-
 iso-propanol 2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ ADH is a putative zinc-dependent alcohol dehydrogenase Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 deduced from amino acid sequence Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q9UXF1
-
-
Saccharolobus solfataricus P2 Q9UXF1
-
-

Purification (Commentary)

Purification (Comment) Organism
HIS-Select High Flow cartridge chromatography Saccharolobus solfataricus

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:ethanol-grown cell highest ethanol consumption rate in cultures grown on 0.79% w/v ethanol Saccharolobus solfataricus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.03
-
 crude extract, using benzylalcohol as substrate, at 65°C Saccharolobus solfataricus
0.18
-
 after 5.67fold purification, using benzylalcohol as substrate, at 65°C Saccharolobus solfataricus
0.75
-
 purified enzyme, using benzylalcohol as substrate, at 80°C Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzylalcohol + NAD+
-
 Saccharolobus solfataricus benzaldehyde + NADH + H+
-
 ?
benzylalcohol + NAD+
-
 Saccharolobus solfataricus P2 benzaldehyde + NADH + H+
-
 ?
ethanol + NAD+
-
 Saccharolobus solfataricus acetaldehyde + NADH + H+
-
 ?
ethanol + NAD+
-
 Saccharolobus solfataricus P2 acetaldehyde + NADH + H+
-
 ?
iso-propanol + NAD+
-
 Saccharolobus solfataricus isopropanal + NADH + H+
-
 ?
iso-propanol + NAD+
-
 Saccharolobus solfataricus P2 isopropanal + NADH + H+
-
 ?
n-propanol + NAD+
-
 Saccharolobus solfataricus propanal + NADH + H+
-
 ?
n-propanol + NAD+
-
 Saccharolobus solfataricus P2 propanal + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
ADH2
-
 Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
 activity increases with temperature up to 95°C Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Saccharolobus solfataricus