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Literature summary for 1.1.1.1 extracted from
- Characterizing NAD-dependent alcohol dehydrogenase enzymes of Methylobacterium extorquens and strawberry (Fragaria x ananassa cv. Elsanta) (2006), J. Agric. Food Chem., 54, 235-242.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 5 mM, 95% inhibition | Fragaria x ananassa |  |
| Ba2+ | 5 mM, 94% inhibition | Methylorubrum extorquens | |
| Cr3+ | 5 mM, 88% loss of activity | Fragaria x ananassa | |
| Cr3+ | 5 mM, 84% loss of activity | Methylorubrum extorquens | |
| EDTA | 25% inhibition at 10.5 mM, 44% inhibition at 21 mM | Fragaria x ananassa | |
| EDTA | 31% inhibition at 10.5 mM, 92% inhibition at 21 mM | Methylorubrum extorquens | |
| Fe2+ | 5 mM, 61% inhibition | Fragaria x ananassa | |
| Fe2+ | 5 mM, 48% inhibition | Methylorubrum extorquens | |
| Li+ | 5 mM, 32% inhibition | Fragaria x ananassa | |
| Li+ | 5 mM, 28% inhibition | Methylorubrum extorquens | |
| Pb2+ | 5 mM, 96% inhibition | Fragaria x ananassa | |
| Pb2+ | 5 mM, 98% inhibition | Methylorubrum extorquens | |
| Zn2+ | 5 mM, 17% loss of activity | Fragaria x ananassa | |
| Zn2+ | 5 mM, 29% loss of activity | Methylorubrum extorquens | |
| Zr2+ | 5 mM, 72% inhibition | Fragaria x ananassa | |
| Zr2+ | 5 mM, 78% inhibition | Methylorubrum extorquens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.78 | - |
methanol | - |
Methylorubrum extorquens | |
| 1.65 | - |
1,2-propanediol | - |
Methylorubrum extorquens | |
| 2.58 | - |
glycerol | - |
Methylorubrum extorquens | |
| 3.09 | - |
1-propanol | - |
Methylorubrum extorquens | |
| 3.42 | - |
2-propanol | - |
Methylorubrum extorquens | |
| 3.54 | - |
1-propanol | - |
Fragaria x ananassa | |
| 4.46 | - |
formaldehyde | - |
Methylorubrum extorquens | |
| 5.38 | - |
ethanol | - |
Methylorubrum extorquens | |
| 6.66 | - |
ethanol | - |
Fragaria x ananassa | |
| 7.72 | - |
benzyl alcohol | - |
Methylorubrum extorquens | |
| 9.69 | - |
methanol | - |
Fragaria x ananassa | |
| 11.46 | - |
2-propanol | - |
Fragaria x ananassa | |
| 15.84 | - |
1,2-propanediol | - |
Fragaria x ananassa | |
| 18.6 | - |
glycerol | - |
Fragaria x ananassa | |
| 19.93 | - |
1-butanol | - |
Fragaria x ananassa | |
| 19.96 | - |
benzyl alcohol | - |
Fragaria x ananassa | |
| 25.78 | - |
1-butanol | - |
Methylorubrum extorquens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24600 | - |
1 * 24600, SDS-PAGE | Fragaria x ananassa |
| 25000 | - |
gel filtration | Fragaria x ananassa |
| 45000 | - |
gel filtration | Methylorubrum extorquens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fragaria x ananassa | - |
- |
- |
| Methylorubrum extorquens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methylorubrum extorquens |
- |
Fragaria x ananassa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.3 | - |
- |
Methylorubrum extorquens |
| 2.5 | - |
- |
Fragaria x ananassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-propanediol + NAD+ | - |
Fragaria x ananassa | hydroxyacetone + NADH | - |
? | |
| 1,2-propanediol + NAD+ | - |
Methylorubrum extorquens | hydroxyacetone + NADH + H+ | - |
? | |
| 1-butanol + NAD+ | - |
Fragaria x ananassa | butanal + NADH | - |
? | |
| 1-butanol + NAD+ | - |
Methylorubrum extorquens | butanal + NADH + H+ | - |
? | |
| 1-propanol + NAD+ | - |
Fragaria x ananassa | propanal + NADH | - |
? | |
| 1-propanol + NAD+ | - |
Methylorubrum extorquens | propanal + NADH + H+ | - |
? | |
| 2-propanol + NAD+ | - |
Fragaria x ananassa | 2-propanone + NADH | - |
? | |
| 2-propanol + NAD+ | - |
Methylorubrum extorquens | 2-propanone + NADH + H+ | - |
? | |
| benzyl alcohol + NAD+ | - |
Fragaria x ananassa | benzaldehyde + NADH | - |
? | |
| benzyl alcohol + NAD+ | - |
Methylorubrum extorquens | benzaldehyde + NADH + H+ | - |
? | |
| ethanol + NAD+ | - |
Fragaria x ananassa | acetaldehyde + NADH | - |
? | |
| ethanol + NAD+ | - |
Methylorubrum extorquens | acetaldehyde + NADH + H+ | - |
? | |
| glycerol + NAD+ | - |
Methylorubrum extorquens | dihydroxyacetone + NADH + H+ | - |
? | |
| glycerol + NAD+ | - |
Fragaria x ananassa | dihydroxyacetone + NADH + H+ | - |
? | |
| methanol + NAD+ | - |
Methylorubrum extorquens | formaldehyde + NADH + H+ | - |
r | |
| methanol + NAD+ | - |
Fragaria x ananassa | formaldehyde + NADH + H+ | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 45000, SDS-PAGE | Methylorubrum extorquens |
| monomer | 1 * 24600, SDS-PAGE | Fragaria x ananassa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Methylorubrum extorquens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | pH 4.0: about 35% of maximal activity, pH 8.0: about 70% of maximal activity | Methylorubrum extorquens |
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