Application | Comment | Organism |
---|---|---|
synthesis | LSADH catalyzed the enantioselective reduction of some ketones with high enantiomeric excesses: phenyl trifluoromethyl ketone to (S)-1-phenyltrifluoroethanol (>99% e.e.), acetophenone to (R)-1-phenylethanol (99% e.e.), and 2-heptanone to (R)-2-heptanol (>99% e.e.) in the presence of 2-propanol without an additional NADH regeneration system. Therefore, it would be a useful biocatalyst | Leifsonia sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | 0.1 mM, 66% inhibition | Leifsonia sp. | |
FeCl3 | 1 mM, 34% inhibition | Leifsonia sp. | |
HgCl2 | 0.1 mM, 85% inhibition | Leifsonia sp. | |
KCN | 1 mM, 41% inhibition | Leifsonia sp. | |
PbCl2 | 1 mM, 63% inhibition | Leifsonia sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
NADH | 25°C, pH 7.0 | Leifsonia sp. | |
0.12 | - |
NAD+ | 25°C, pH 7.0 | Leifsonia sp. | |
13.6 | - |
phenyl trifluoromethyl ketone | 25°C, pH 7.0 | Leifsonia sp. | |
57.5 | - |
2-propanol | 25°C, pH 7.0 | Leifsonia sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
4 * 26000, SDS-PAGE | Leifsonia sp. |
110000 | - |
gel filtration | Leifsonia sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leifsonia sp. | - |
- |
- |
Leifsonia sp. S749 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Leifsonia sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.3 | - |
- |
Leifsonia sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-butanol + NAD+ | 50% of the activity with 2-propanol | Leifsonia sp. | 2-butanone + NADH + H+ | - |
ir | |
(R)-2-butanol + NAD+ | 50% of the activity with 2-propanol | Leifsonia sp. S749 | 2-butanone + NADH + H+ | - |
ir | |
(R)-2-heptanol + NAD+ | 2469% of the activity with 2-propanol | Leifsonia sp. | 2-heptanone + NADH + H+ | - |
r | |
(R)-2-heptanol + NAD+ | 2469% of the activity with 2-propanol | Leifsonia sp. S749 | 2-heptanone + NADH + H+ | - |
r | |
(R)-2-hexanol + NAD+ | 1906% of the activity with 2-propanol | Leifsonia sp. | 2-hexanone + NADH + H+ | - |
r | |
(R)-2-hexanol + NAD+ | 1906% of the activity with 2-propanol | Leifsonia sp. S749 | 2-hexanone + NADH + H+ | - |
r | |
(R)-2-octanol + NAD+ | 2406% of the activity with 2-propanol | Leifsonia sp. | 2-octanone + NADH + H+ | - |
r | |
(R)-2-octanol + NAD+ | 2406% of the activity with 2-propanol | Leifsonia sp. S749 | 2-octanone + NADH + H+ | - |
r | |
(R)-2-pentanol + NAD+ | 75% of the activity with 2-propanol | Leifsonia sp. | 2-pentanone + NADH + H+ | - |
r | |
(S)-2-butanol + NAD+ | 275% of the activity with 2-propanol | Leifsonia sp. | 2-butanone + NADH + H+ | - |
ir | |
(S)-2-heptanol + NAD+ | 331% of the activity with 2-propanol | Leifsonia sp. | 2-heptanone + NADH + H+ | - |
r | |
(S)-2-hexanol + NAD+ | 38% of the activity with 2-propanol | Leifsonia sp. | 2-hexanone + NADH + H+ | - |
r | |
(S)-2-octanol + NAD+ | 81% of the activity with 2-propanol | Leifsonia sp. | 2-octanone + NADH + H+ | - |
r | |
(S)-2-pentanol + NAD+ | 25% of the activity with 2-propanol | Leifsonia sp. | 2-pentanone + NADH + H+ | - |
r | |
1,1-dichloroacetone + NADH + H+ | 1078% of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | 1,1-dichloropropan-2-ol + NAD+ | - |
? | |
1-heptanol + NAD+ | 56% of the activity with 2-propanol, in the reverse reaction 755% of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | heptanal + NADH + H+ | - |
r | |
1-hexanol + NAD+ | 44% of the activity with 2-propanol, in the reverse reaction 1029% of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | hexanal + NADH + H+ | - |
r | |
1-octanol + NAD+ | 33% of the activity with 2-propanol, in the reverse reaction 435% of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | octanal + NADH + H+ | - |
r | |
1-phenyl-2-butanone + NADH + H+ | 12% of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | 1-phenylbutan-2-ol + NAD+ | - |
? | |
1-phenyl-3-butanone + NADH + H+ | 353% of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | 1-phenylbutan-3-ol + NAD+ | - |
? | |
2,3'-dichloroacetophenone + NADH + H+ | 67% of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | 2-chloro-1-(3-chlorophenyl)ethanol + NAD+ | - |
? | |
2-heptanone + NADH + H+ | 229% of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | (R)-2-heptanol + NAD+ | 99% enantiomeric excess | ? | |
2-propanol + NAD+ | - |
Leifsonia sp. | acetone + NADH + H+ | - |
ir | |
3',4'-dimethoxyphenylacetone + NADH + H+ | 24% of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | 1-(3,4-dimethoxyphenyl)propan-2-ol + NAD+ | - |
? | |
3'-bromoacetophenone + NADH + H+ | 151% of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | 1-(3-bromophenyl)ethanol + NAD+ | - |
? | |
3'-chloroacetophenone + NADH + H+ | 70% of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | 1-(3-chlorophenyl)ethanol + NAD+ | - |
? | |
3'-methoxyacetophenone + NADH + H+ | 51% of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | 1-(3-methoxyphenyl)ethanol + NAD+ | - |
? | |
3-chloro-2-butanone + NADH + H+ | 151% of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | 3-chlorobutan-2-ol + NAD+ | - |
? | |
3-heptanol + NAD+ | 93% of the activity with 2-propanol | Leifsonia sp. | 3-heptanone + NADH + H+ | - |
r | |
3-phenylpropionaldehyde + NADH | 1218% of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | 3-phenylpropan-1-ol + NAD+ | - |
? | |
4'-bromoacetophenone + NADH + H+ | 77% of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | 1-(4-bromophenyl)ethanol + NAD+ | - |
? | |
4'-chloroacetophenone + NADH + H+ | 60% of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | 1-(4-chlorophenyl)ethanol + NAD+ | - |
? | |
4-hydroxy-2-butanone + NADH + H+ | 26% of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | 2,4-dihydroxybutane + NAD+ | - |
? | |
acetophenone + NADH + H+ | 6% of the activity with phenyl trifluoromethyl ketone, in the reverse reaction 87% of the activity with 2-propanol | Leifsonia sp. | (R)-1-phenylethanol + NAD+ | 99% enantiomeric excess | ? | |
butanal + NADH | % of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | 1-butanol + NAD+ + H+ | - |
ir | |
chloroacetone + NADH + H+ | 238% of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | 1-chloropropan-2-ol + NAD+ | - |
? | |
ethyl 3-methyl-2-oxobutyrate + NADH + H+ | 33% of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | ethyl-2-hydroxy-3-methylbutyrate + NAD+ | - |
? | |
ethyl 3-oxobutanoate + NADH + H+ | 309% of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | ethyl 3-hydroxybutanoate + NAD+ | - |
? | |
ethyl 4-bromo-3-oxobutanoate + NADH + H+ | 511% of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | ethyl 4-bromo-3-hydroxybutanoate | - |
? | |
ethyl 4-chloro-3-oxobutanoate + NADH + H+ | 809% of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | ethyl 4-chloro-3-hydroxybutanoate + NAD+ | - |
? | |
ethyl pyruvate + NADH + H+ | 488% of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | ethyl 2-hydroxypropanoate + NAD+ | - |
? | |
methyl 3-oxobutanoate + NADH + H+ | 130% of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | methyl 3-hydroxybutanoate + NAD+ | - |
? | |
methyl 4-bromo-3-oxobutanoate + NADH + H+ | 164% of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | methyl 4-bromo-3-hydroxybutanoate + NAD+ | - |
? | |
additional information | the enzyme does not act on short-chain normal alkyl alcohols, including methanol and ethanol | Leifsonia sp. | ? | - |
? | |
additional information | the enzyme does not act on short-chain normal alkyl alcohols, including methanol and ethanol | Leifsonia sp. S749 | ? | - |
? | |
pentanal + NADH | 132% of the activity with phenyl trifluoromethyl ketone, in the reverse reaction 5% of the activity with 2-propanol | Leifsonia sp. | 1-pentanol + NAD+ + H+ | - |
? | |
phenyl trifluoromethyl ketone + NADH + H+ | - |
Leifsonia sp. | (S)-1-phenyltrifluoroethanol + NAD+ | more than 99% enantiomeric excess | ? | |
tert-butyl 3-oxobutanoate + NADH + H+ | 568% of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | tert butyl 3-hydroxybutanoate + NAD+ | - |
? | |
trans-cinnamaldehyde + NADH + H+ | % of the activity with phenyl trifluoromethyl ketone | Leifsonia sp. | cinnamyl alcohol + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 26000, SDS-PAGE | Leifsonia sp. |
Synonyms | Comment | Organism |
---|---|---|
LSADH | - |
Leifsonia sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Leifsonia sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | 20°C: 23% of maximal activity, 70°C: 40% of maximal activity | Leifsonia sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
1 h in 20 mM Tris-HCl buffer, pH 7.0, stable below | Leifsonia sp. |
40 | - |
1 h in 20 mM Tris-HCl buffer, pH 7.0, retains 80% of its activity | Leifsonia sp. |
50 | - |
1 h in 20 mM Tris-HCl buffer, pH 7.0, retains 70% of its activity | Leifsonia sp. |
60 | - |
1 h in 20 mM Tris-HCl buffer, pH 7.0, retains 24% of its activity | Leifsonia sp. |
70 | - |
1 h in 20 mM Tris-HCl buffer, pH 7.0, complete loss of activity | Leifsonia sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
reductive reaction | Leifsonia sp. |
9.5 | - |
oxidative reaction | Leifsonia sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9.5 | 25°C, 1 h, stable | Leifsonia sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Leifsonia sp. | |
NADH | - |
Leifsonia sp. | |
NADPH | weak activity | Leifsonia sp. |