Any feedback?
Literature summary for 1.1.1.1 extracted from
- The ternary complex of Pseudomonas aeruginosa alcohol dehydrogenase with NADH and ethylene glycol (2004), Protein Sci., 13, 1547-1556.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli strain TG-1 | Pseudomonas aeruginosa |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| ternary complex of enzyme with NADH and ethylene glycol, X-ray diffraction structure determination and analysis at 2.3 A resolution, molecular replacement method | Pseudomonas aeruginosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 200 | - |
ethylene glycol | above | Pseudomonas aeruginosa |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | 1 catalytic and 1 structural zinc ion per subunit, coordination complex geometry | Pseudomonas aeruginosa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
PaADH | - |
| Pseudomonas aeruginosa PaADH | - |
PaADH | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| a primary alcohol + NAD+ = an aldehyde + NADH + H+ | the catalytic triad consists of Cys44, His67, and Cys154, active site structure | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethylene glycol + NAD+ | poor substrate | Pseudomonas aeruginosa | ? | - |
r | |
| ethylene glycol + NAD+ | poor substrate | Pseudomonas aeruginosa PaADH | ? | - |
r | |
| primary or secondary alcohol + NAD+ | - |
Pseudomonas aeruginosa | aldehyde or ketone + NADH | - |
r | |
| primary or secondary alcohol + NAD+ | - |
Pseudomonas aeruginosa PaADH | aldehyde or ketone + NADH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | quaternary organization and stability, overview | Pseudomonas aeruginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADH | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
assay at | Pseudomonas aeruginosa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.5 | - |
ethylene glycol | - |
Pseudomonas aeruginosa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | - |
assay at | Pseudomonas aeruginosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pseudomonas aeruginosa | |
| NADH | - |
Pseudomonas aeruginosa | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
