Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.1 extracted from

  • Miroliaei, M.; Nemat-Gorgani, M.
    Effect of organic solvents on stability and activity of two related alcohol dehydrogenases: a comparative study (2002), Int. J. Biochem. Cell Biol., 34, 169-175.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Thermoanaerobacter brockii

Organic Solvent Stability

Organic Solvent Comment Organism
dimethylformamide 50% v/v, 20% remaining activity at 4°C or 25°C after 1-100 min Thermoanaerobacter brockii
dimethylformamide 50% v/v, 75% remaining activity at 4°C after 100 min, 15% remaining activity after 100 min at 25°C Saccharomyces cerevisiae
dioxane 50% v/v, 50% remaining activity at 4°C after 100 min, 10% remaining activity after 100 min at 25°C Saccharomyces cerevisiae
dioxane 50% v/v, about 95% remaining activity at 4°C or 25°C after 1-100 min Thermoanaerobacter brockii
dodecane active, from 25°C up to 75°C, 3 h, stabilization of the enzyme at elevated temperature Saccharomyces cerevisiae
dodecane active, from 25°C up to 90°C, 3 h Thermoanaerobacter brockii
octane active, from 25°C up to 75°C, 3 h, stabilization of the enzyme at elevated temperature Saccharomyces cerevisiae
octane active, from 25°C up to 90°C, 3 h Thermoanaerobacter brockii
Pyridine inactivation Saccharomyces cerevisiae
Pyridine active, from 25°C up to 90°C, 3 h Thermoanaerobacter brockii
toluene active, from 25°C up to 75°C, 3 h, stabilization of the enzyme at elevated temperature Saccharomyces cerevisiae
toluene active, from 25°C up to 90°C, 3 h Thermoanaerobacter brockii

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Thermoanaerobacter brockii
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Saccharomyces cerevisiae
-
commercial preparation
-
Thermoanaerobacter brockii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-propanol + NAD(P)+
-
Saccharomyces cerevisiae acetone + NAD(P)H
-
?
2-propanol + NAD(P)+
-
Thermoanaerobacter brockii acetone + NAD(P)H
-
?
ethanol + NAD+
-
Saccharomyces cerevisiae acetaldehyde + NADH
-
?
ethanol + NAD+
-
Thermoanaerobacter brockii acetaldehyde + NADH
-
?

Synonyms

Synonyms Comment Organism
TBADH
-
Thermoanaerobacter brockii
YADH
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Saccharomyces cerevisiae
65
-
assay at Thermoanaerobacter brockii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable up to, about 20% remaining activity after 3 h Saccharomyces cerevisiae
75
-
inactivation Saccharomyces cerevisiae
90
-
stable up to Thermoanaerobacter brockii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Saccharomyces cerevisiae
7.8
-
assay at Thermoanaerobacter brockii

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Saccharomyces cerevisiae
NAD+
-
Thermoanaerobacter brockii
NADP+
-
Saccharomyces cerevisiae
NADP+
-
Thermoanaerobacter brockii