Any feedback?
Literature summary for 1.1.1.1 extracted from
- The highly stable alcohol dehydrogenase of Thermomicrobium roseum: purification and molecular characterization (2002), Comp. Biochem. Physiol. B, 132, 415-422.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetaldehyde | strong product inhibition | Thermomicrobium roseum |  |
| imidazole | weak inhibition | Thermomicrobium roseum | |
| pyrazole | strong inhibition | Thermomicrobium roseum | |
| Pyridine | - |
Thermomicrobium roseum | |
| trichloroethanol | weak inhibition | Thermomicrobium roseum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 24.2 | - |
ethanol | pH 10.0, 50°C | Thermomicrobium roseum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
2 * 43000, SDS-PAGE | Thermomicrobium roseum |
| 88000 | - |
approximately, gel filtration | Thermomicrobium roseum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermomicrobium roseum | - |
ATCC 27502 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 128fold to homogeneity by adenosine 5'-monophosphate affinity and Mono-Q ion exchange chromatography | Thermomicrobium roseum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.28 | - |
purified enzyme | Thermomicrobium roseum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2,2-trichloroethanol + NAD+ | - |
Thermomicrobium roseum | trichloroacetaldehyde + NADH + H+ | - |
? | |
| crotyl alcohol + NAD+ | - |
Thermomicrobium roseum | crotonaldehyde + NADH | - |
? | |
| ethanol + NAD+ | best substrate | Thermomicrobium roseum | acetaldehyde + NADH | - |
r | |
| methanol + NAD+ | low activity | Thermomicrobium roseum | formaldehyde + NADH + H+ | - |
? | |
| additional information | enzyme shows high substrate specificity towards primary aliphatic alcohols, no activity with 2-butanol, tert-butanol, isoamyl alcohol, isobutyl alcohol, 1,6-hexadiol, and mono-, di-, and triethanolamine | Thermomicrobium roseum | ? | - |
? | |
| n-butanol + NAD+ | low activity | Thermomicrobium roseum | butanal + NADH | - |
? | |
| n-propanol + NAD+ | - |
Thermomicrobium roseum | propionaldehyde + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 43000, SDS-PAGE | Thermomicrobium roseum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADH | - |
Thermomicrobium roseum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Thermomicrobium roseum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 85 | maximal activity at 70°C, 60% of maximal activity at 85°C | Thermomicrobium roseum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
stable upt to | Thermomicrobium roseum |
| 75 | - |
and above, rapid loss of activity | Thermomicrobium roseum |
| 85 | - |
slight residual activity | Thermomicrobium roseum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Thermomicrobium roseum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Thermomicrobium roseum | |
| NADH | - |
Thermomicrobium roseum | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Thermomicrobium roseum | - |
- |
6 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
